Locked nucleic acid: A potent nucleic acid analog in therapeutics and biotechnology

被引：141

作者：

Jepsen, JS

Sorensen, MD

Wengel, J

机构：

[1] Danish Canc Soc, Dept Tumor Endocrinol, Inst Canc Biol, DK-2100 Copenhagen, Denmark

[2] Univ Copenhagen, Dept Chem, DK-2100 Copenhagen, Denmark

[3] Univ So Denmark, Nucl Acid Ctr, Dept Chem, DK-5230 Odense M, Denmark

来源：

OLIGONUCLEOTIDES | 2004年 / 14卷 / 02期

关键词：

D O I：

10.1089/1545457041526317

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Locked nucleic acid (LNA) is a class of nucleic acid analogs possessing very high affinity and excellent specificity toward complementary DNA and RNA, and LNA oligonucleotides have been applied as antisense molecules both in vitro and in vivo. In this review, we briefly describe the basic physiochemical properties of LNA and some of the difficulties that may be encountered when applying LNA technology. The central part of the review focuses on the use of LNA molecules in regulation of gene expression, including delivery to cells, stability, unspecific effects, toxicity, pharmacokinetics, and design of LNA oligonucleotides. The last part evaluates LNA as a diagnostic tool in genotyping.

引用

收藏

页码：130 / 146

页数：17

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