Large-scale identification of differentially expressed genes during neurogenesis

被引：4

作者：

Pazman, C

Castelli, JC

Wen, XL

Somogyi, R

机构：

[1] NINDS, Neurophysiol Lab, Bethesda, MD 20892 USA

[2] Univ Calif San Francisco, Dept Med, San Francisco, CA USA

来源：

NEUROREPORT | 2000年 / 11卷 / 04期

关键词：

differential display; NGF; PC12; spinal cord;

D O I：

10.1097/00001756-200003200-00013

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We report here a modified mRNA differential display method and its application for the analysis of differential gene expression in NGF-treated PC12 cells and in embryonic rat spinal cord. The optimized protocol is based on low fidelity priming of multiple cDNAs followed by high fidelity amplification. In PC12 cells induction by nerve growth factor (NGF) altered the expression of 4% of the 466 transcripts evaluated. During neurogenesis of the spinal cord we found that 30% of the 288 examined products changed. The differential expression of the characterized genes was confirmed by independent quantitative PCR. We conclude this method is suitable for the identification of increases and decreases of mRNA levels and allows the discovery of differentially expressed unknown transcripts. NeuroReport 11:719-724 (C) 2000 Lippincott Williams & Wilkins.

引用

收藏

页码：719 / 724

页数：6

相关论文

共 20 条

[1] IDENTIFICATION OF MULTIPLE GENES IN BOVINE RETINAL PERICYTES ALTERED BY EXPOSURE TO ELEVATED LEVELS OF GLUCOSE BY USING MESSENGER-RNA DIFFERENTIAL DISPLAY [J].

AIELLO, LP ;

ROBINSON, GS ;

LIN, YW ;

NISHIO, Y ;

KING, GL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (13) :6231-6235

[2] A novel seizure-induced synaptotagmin gene identified by differential display [J].

Babity, JM ;

Armstrong, JN ;

Plumier, JCL ;

Currie, RW ;

Robertson, HA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (06) :2638-2641

[3] IDENTIFICATION OF DIFFERENTIALLY EXPRESSED MESSENGER-RNA SPECIES BY AN IMPROVED DISPLAY TECHNIQUE (DDRT-PCR) [J].

BAUER, D ;

MULLER, H ;

REICH, J ;

RIEDEL, H ;

AHRENKIEL, V ;

WARTHOE, P ;

STRAUSS, M .

NUCLEIC ACIDS RESEARCH, 1993, 21 (18) :4272-4280

[4] Differential display of genome subsets containing specific interspersed repeats [J].

Broude, NE ;

Chandra, A ;

Smith, CL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (09) :4548-4553

[5] Rational primer design greatly improves differential display PCR (DD-PCR) [J].

Graf, D ;

Fisher, AG ;

Merkenschlager, M .

NUCLEIC ACIDS RESEARCH, 1997, 25 (11) :2239-2240

[6]

HALEGOUA S, 1991, CURR TOP MICROBIOL, V165, P119

[7] EXTRACELLULAR SIGNALS, TRANSCRIPTIONAL RESPONSES AND CELLULAR SPECIFICITY [J].

HERSCHMAN, HR .

TRENDS IN BIOCHEMICAL SCIENCES, 1989, 14 (11) :455-458

[8] DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION [J].

LIANG, P ;

PARDEE, AB .

SCIENCE, 1992, 257 (5072) :967-971

[9] DISTRIBUTION AND CLONING OF EUKARYOTIC MESSENGER-RNAS BY MEANS OF DIFFERENTIAL DISPLAY - REFINEMENTS AND OPTIMIZATION [J].

LIANG, P ;

AVERBOUKH, L ;

PARDEE, AB .

NUCLEIC ACIDS RESEARCH, 1993, 21 (14) :3269-3275

[10] DIFFERENTIAL DISPLAY USING ONE-BASE ANCHORED OLIGO-DT PRIMERS [J].

LIANG, P ;

ZHU, WM ;

ZHANG, XY ;

GUO, Z ;

OCONNELL, RP ;

AVERBOUKH, L ;

WANG, FL ;

PARDEE, AB .

NUCLEIC ACIDS RESEARCH, 1994, 22 (25) :5763-5764