Aldehyde dehydrogenase 1 is a putative marker for cancer stem cells in head and neck squamous cancer

被引：435

作者：

Chen, Yu-Chih

Chen, Yi-Wei ^{[3
]}

Hsu, Han-Shui ^{[4
]}

Tseng, Ling-Ming ^{[4
]}

Huang, Pin-I ^{[3
]}

Lu, Kai-Hsi ^{[5
]}

Chen, Dow-Tien ^{[5
]}

Tai, Lung-Kuo ^{[5
]}

Yung, Ming-Chi ^{[6
]}

Chang, Shih-Ching ^{[4
]}

Ku, Hung-Hai ^{[6
]}

Chiou, Shih-Hwa ^{[2
,5
]}

Lo, Wen-Liang ^{[1
,2
]}

机构：

[1] Taipei Vet Gen Hosp, Div Oral & Maxillofacial Surg, Taipei 11217, Taiwan

[2] Natl Yang Ming Univ, Dept Dent, Taipei 112, Taiwan

[3] Taipei Vet Gen Hosp, Ctr Canc, Taipei, Taiwan

[4] Taipei Vet Gen Hosp, Dept Surg, Taipei, Taiwan

[5] Taipei Vet Gen Hosp, Dept Med Res & Educ, Taipei, Taiwan

[6] Taipei City Hosp, Zhongxiao Branch, Dept Cardiovasc Surg, Dept Med Educ & Res, Taipei, Taiwan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 385卷 / 03期

关键词：

Aldehyde dehydrogenase 1; Cancer stem cell; Head and neck squamous cancer; EPITHELIAL-MESENCHYMAL TRANSITION; LUNG-CANCER; CARCINOMA; EXPRESSION; IDENTIFICATION; METASTASIS; SNAIL; OCT-4;

D O I：

10.1016/j.bbrc.2009.05.048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aldehyde dehydrogenase 1 (ALDH1) has been considered to be a marker for cancer stern cells. However, the role of ALDH1 in head and neck squamous cell carcinoma (HNSCC) has yet to be determined. In this study, we isolated ALDH1-positive cells from HNSCC patients and showed that these HNSCC-ALDH1(+) cells displayed radioresistance and represented a reservoir for generating tumors. Based on microarray findings, the results of Western blotting and immunofluorescent assays further confirmed that ALDH1(+)-line-age cells showed evidence of having epithelial-mesenchymal transition (EMT) shifting and endogenously co-expressed Snail. Furthermore, the knockdown of Snail expression significantly decreased the expression of ALDH1, inhibited cancer stern-like properties, and blocked the tumorigenic abilities of CD44(+)CD24(-)ALDH1(+) cells. Finally, in a xenotransplanted tumorigenicity study, we confirmed that the treatment effect of chemoradiotherapy for ALDH1(+) could be improved by Snail siRNA. In summary, it is likely that ALDH1 is a specific marker for the cancer stern-like cells of HNSCC. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：307 / 313

页数：7

共 24 条

[1] Phenotypic characterization of murine primitive hematopoietic progenitor cells isolated on basis of aldehyde dehydrogenase activity [J].