Human neuronal tau promoting the melting temperature of DNA

被引：15

作者：

Hua, Q ^{[1
]}

He, RQ ^{[1
]}

机构：

[1] Acad Sinica, Inst Biophys, Lab Visual Informat Proc, Beijing 100101, Peoples R China

来源：

CHINESE SCIENCE BULLETIN | 2000年 / 45卷 / 11期

基金：

中国国家自然科学基金;

关键词：

human neuronal tau; DNA; Tm of DNA; double helix of DNA; denaturation;

D O I：

10.1007/BF02884979

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 [理学]; 0710 [生物学]; 09 [农学];

摘要：

The hyperchromic effect of ultraviolet spectroscopy shows that adding recombinant human neuronal tau to the solution of calf thymus DNA will promote the melting temperature (T-m) from 67 degrees C to 81 degrees C, Similar result has been detected when adding tau to plasmid pBluescript-II SK, by raising Tm from 75 degrees C to 85 degrees C. The kinetics of thermal denaturation of DNA with tau is much slower than that of control. It suggests that tau may stabilize the double helix conformation of DNA.

引用

页码：999 / 1002

页数：4

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