Molecular characterization and species identification of the Anopheles dirus and An. minimus complexes in north-east India using r-DNA ITS-2

被引：33

作者：

Prakash, Anil

Walton, C.

Bhattacharyya, D. R.

O'Loughlin, Samantha

Mohapatra, P. K.

Mahanta, J.

机构：

[1] NE Indian Council Med Res, Reg Med Res Ctr, Dibrugarh 786001, Assam, India

[2] Univ Manchester, Fac Life Sci, Manchester M13 9PT, Lancs, England

来源：

ACTA TROPICA | 2006年 / 100卷 / 1-2期

关键词：

Anopheles baimaii; Anopheles minimus; molecular identification; north-east India; r-DNA ITS2; POLYMERASE-CHAIN-REACTION; RIBOSOMAL DNA; CULICIDAE; DIPTERA; MEMBERS; ASIA;

D O I：

10.1016/j.actatropica.2006.09.009

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The sibling species composition of the Anopheles minimus and Anopheles dirus complexes is poorly known in the highly malarious north-eastern region of India where these two vector taxa are accountable for most of the malaria transmission among 30.7 million inhabitants. Prevalent members of these two complexes in this part of India were identified using sequences for the second internal transcribed spacer (ITS2) of ribosomal DNA. Anopheles baimaii (species D) of the An. dirus complex and An. minimus s.s. (species A) of the An. minimus complex were detected in Arunachal Pradesh, Assam, Meghalaya and Nagaland states. No intraspecific variation was observed in the ITS2 sequence (479 bp) of An. baimaii whereas a single substitution was detected in the ITS2 sequence (372 bp) of An. minimus from Nagaland state. (c) 2006 Published by Elsevier B.V.

引用

页码：156 / 161

页数：6

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