Exosomes from human saliva as a source of microRNA biomarkers

被引：606

作者：

Michael, A. ^{[1
]}

Bajracharya, S. D. ^{[1
]}

Yuen, P. S. T. ^{[2
]}

Zhou, H. ^{[2
]}

Star, R. A. ^{[2
]}

Illei, G. G. ^{[1
]}

Alevizos, I. ^{[1
]}

机构：

[1] Natl Inst Dent & Craniofacial Res, Sjogrens Syndrome Clin, Mol Physiol & Therapeut Branch, Bethesda, MD 20892 USA

[2] NIDDKD, Renal Diagnost & Therapeut Unit, Bethesda, MD 20892 USA

来源：

ORAL DISEASES | 2010年 / 16卷 / 01期

关键词：

salivary exosomes; microRNA; biomarkers; DIAGNOSTIC BIOMARKERS; URINARY EXOSOMES; PROSTATE-CANCER; CELLS; RNAS; MICROVESICLES; IMMUNOLOGY; EXPRESSION; SIGNATURES; PROTEINS;

D O I：

10.1111/j.1601-0825.2009.01604.x

中图分类号：

R78 [口腔科学];

学科分类号：

100302 [口腔临床医学];

摘要：

Objective: The aim of this study was to examine the presence of microRNAs (miRNAs) within exosomes isolated from human saliva and to optimize and test methods for successful downstream applications. Design: Exosomes isolated from fresh and frozen glandular and whole human saliva were used as a source of miRNAs. The presence of miRNAs was validated with TaqMan quantitative PCR and miRNA microarrays. Results: We successfully isolated exosomes from human saliva from healthy controls and a patient with Sjogren's syndrome. microRNAs extracted from the exosomal fraction were sufficient for quantitative PCR and microarray profiling. Conclusions: The isolation of miRNAs from easily and non-invasively obtained salivary exosomes with subsequent characterization of the miRNA expression patterns is promising for the development of future biomarkers of the diagnosis and prognosis of various salivary gland pathologies.

引用

页码：34 / 38

页数：5

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