Molecular typing of novel Rickettsia rickettsii isolates from Arizona

被引：23

作者：

Eremeeva, Marina E. ^{[1
]}

Bosserman, Elizabeth ^{[1
]}

Zambrano, Maria ^{[1
]}

Demma, Linda ^{[1
]}

Dasch, Gregory A. ^{[1
]}

机构：

[1] CDC, Viral & Rickettsial Zoonoses Branch, Atlanta, GA 30333 USA

来源：

CENTURY OF RICKETTSIOLOGY: EMERGING, REEMERGING RICKETTSIOSES, MOLECULAR DIAGNOSTICS, AND EMERGING VETERINARY RICKETTSIOSES | 2006年 / 1078卷

关键词：

Rickettsia rickettsii; VNTR; molecular marker;

D O I：

10.1196/annals.1374.114

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Seven isolates of Rickettsia rickettsii were obtained from a skin biopsy, two whole-blood specimens, and from Rhipicephalus sanguineus ticks from eastern Arizona. Molecular typing of seven isolates of R. rickettsii and DNA samples from two other Rh. sanguineus ticks infected with R. rickettsii was conducted by PCR and DNA sequencing of rompA and 12 variable-number tandem repeat regions (VNTRs). All DNA specimens from Arizona were identical to each other and to reference human and Dermacentor andersoni isolates of R. rickettsii from Montana in their rOmpA gene sequences and 10 VNTRs. Two of the twelve VNTRs had differences in the number of repeat sequences in isolates from Arizona compared to those from Montana, thus conferring the novelty of the Rh. sanguines-associated R. rickettsii.

引用

页码：573 / 577

页数：5

共 4 条

[1] Rocky Mountain spotted fever in the United States, 1997-2002 [J].

Chapman, Alice S. ;

Murphy, Staci M. ;

Demma, Linda J. ;

Holman, Robert C. ;

Curns, Aaron T. ;

McQuiston, Jennifer H. ;

Krebs, John W. ;

Swerdlow, David L. .

VECTOR-BORNE AND ZOONOTIC DISEASES, 2006, 6 (02) :170-178

[2] Rocky mountain spotted fever from an unexpected tick vector in Arizona [J].

Demma, LJ ;

Traeger, MS ;

Nicholson, WL ;

Paddock, CD ;

Blau, DM ;

Eremeeva, ME ;

Dasch, GA ;

Levin, ML ;

Singleton, JJ ;

Zaki, SR ;

Cheek, JE ;

Swerdlow, DL ;

McQuiston, JH .

NEW ENGLAND JOURNAL OF MEDICINE, 2005, 353 (06) :587-594

[3]

EREMEEVA M, 2004, ASM 2004 BIOD M BALT

[4] Genetic analysis of isolates of Rickettsia rickettsii that differ in virulence [J].

Eremeeva, ME ;

Klemt, RM ;

Santucci-Domotor, LA ;

Silverman, DJ ;

Dasch, GA .

RICKETTSIOLOGY: PRESENT AND FUTURE DIRECTIONS, 2003, 990 :717-722

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