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Electrochemical DNA biosensor for the detection of DNA hybridization with the amplification of Au nanoparticles and CdS nanoparticles

被引：108
作者：
Du, Ping [1 ]
Li, Hongxia [1 ]
Mei, Zhenhua [1 ]
Liu, Shufeng [1 ]
机构：
[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Minist Educ, Key Lab Ecochem Engn, Qingdao 266042, Peoples R China
来源：
BIOELECTROCHEMISTRY | 2009年 / 75卷 / 01期
关键词：
Au nanoparticles; CdS nanoparticles; Voltammetry; DNA hybridization; DNA biosensor; SURFACE-PLASMON RESONANCE; GOLD NANOPARTICLES; CARBON NANOTUBES; SENSITIVITY; DEPOSITION; MORPHOLOGY; SUBSTRATE; FILMS;
D O I：
10.1016/j.bioelechem.2009.01.003
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this article. colloidal gold nanoparticles (Au NPs) and carboxyl group-functionalized CdS Nanoparticles (CdS NPs) were immobilized on the Au electrode surface to fabricate a novel electrochemical DNA biosensor. Both Au NPs and CdS NPs, well known to be good biocompatible and conductive materials, could provide larger surface area and sufficient amount of binding points for DNA immobilization. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) experiments were performed to follow the whole electrode fabrication process. DNA immobilization and hybridization were characterized with differential pulse voltammetry (DPV) by using [Co(phen)(2)(Cl)(H2O)]Cl center dot 2H(2)O as an electrochemical hybridization indicator. With this approach, the target DNA could be quantified at a linear range from 2.0x10(-10) to 1.0x1.0(-8) M, with a detection limit of 2.0x10(-11) M by 3 sigma. In addition, the biosensor exhibited a good repeatability and stability for the determination of DNA sequences. (c) 2009 Elsevier B.V. All rights reserved.
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收藏
页码：37 / 43
页数：7
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