Liquid chromatographic human serum acid and mass spectrometric analysis of alpha-1-glycoprotein

被引：31

作者：

Kremmer, T

Szöllösi, É

Boldizsár, M

Vincze, B

Ludányi, K

Imre, T

Schlosser, G

Vékey, K

机构：

[1] Natl Inst Oncol, Dept Biochem, H-1122 Budapest, Hungary

[2] Hungarian Acad Sci, Chem Res Ctr, H-1525 Budapest, Hungary

来源：

BIOMEDICAL CHROMATOGRAPHY | 2004年 / 18卷 / 05期

关键词：

orosomucoid; oligosaccharicle; RP-HPLC; MALDI-TOF MS;

D O I：

10.1002/bmc.324

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Human serum acid alpha-l-glycoprotein (AGP, orosomucoid) content of healthy individuals and cancer patients was measured, isolated and purified using a protocol of fast and biocompatible sample preparation, ion exchange and dye-ligand affinity chromatographic methods. In comparison to the healthy individuals significantly higher serum AGP levels were found in a wide spectrum of cancer patients, indicating its diagnostic value in the malignant disease. Oligosaccharide content of AGP samples was separated following PNGase F enzyme digestion and analysed by RP-HPLC and MALDI-TOF mass spectrometry. RP-HPLC and MALDI-TOF mass spectrometric analysis of sugar constituents of AGP specimen originated from selected cancer patients with high serum AGP levels indicated the appearance of anomal distribution of bi-, tri- and tetra-antennary oligosaccharide structures compared to the healthy controls. Copyright (C) 2004 John Wiley Sons, Ltd.

引用

页码：323 / 329

页数：7

共 35 条

[1]

Characterization of carbohydrates using highly fluorescent 2-aminobenzoic acid tag following gel electrophoresis of glycoproteins [J].