Biotransformation of Ginsenoside Rf to Rh1 by Recombinant β-Glucosidase

被引：35

作者：

Ruan, Chang-Chun ^{[2
,3
]}

Zhang, Hao ^{[4
]}

Zhang, Lian-Xue ^{[2
]}

Liu, Zhi ^{[3
]}

Sun, Guang-Zhi ^{[3
]}

Lei, Jun ^{[2
]}

Qin, Yu-Xia ^{[5
]}

Zheng, Yi-Nan ^{[2
]}

Li, Xiang ^{[1
]}

Pan, Hong-Yu ^{[6
]}

机构：

[1] Agr & Agri Food Canada, Saskatoon Res Ctr, Saskatoon, SK S7N 0X2, Canada

[2] Jilin Agr Univ, Coll Chinese Med Mat, Changchun 130118, Peoples R China

[3] Jilin Agr Univ, Inst Agr Modernizat, Changchun 130118, Peoples R China

[4] Jilin Agr Univ, Sch Resources & Environm, Changchun 130118, Peoples R China

[5] Jilin Agr Univ, Engn Res Ctr Bioreactor & Pharmaceut Dev, Minist Educ, Changchun 130118, Peoples R China

[6] Jilin Univ, Coll Plant Sci, Changchun 130062, Peoples R China

来源：

MOLECULES | 2009年 / 14卷 / 06期

关键词：

Aspergillus niger; beta-glucosidase; ginsenoside Rf; ginsenoside Rh-1; SK-HEP-1; CELLS; PANAX-GINSENG; SAPONINS;

D O I：

10.3390/molecules14062043

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An Aspergillus niger strain was isolated from the soil around ginseng fruit. In vitro enzyme assays showed that this strain had the ability to transform total ginsenosides (TGS) into several new products. In a further biochemical study, a beta-glucosidase gene isolated from this strain, bgl1, was expressed in Saccharomyces cerevisiae. His-tagged BGL1 protein (similar to 170 kD) showed the ability to transform ginsenoside Rf into Rh-1.

引用

页码：2043 / 2048

页数：6

共 12 条

[1] Intestinal bacterial hydrolysis is required for the appearance of compound K in rat plasma after oral administration of ginsenoside Rb1 from Panax ginseng [J].

Akao, T ;

Kida, H ;

Kanaoka, M ;

Hattori, M ;

Kobashi, K .

JOURNAL OF PHARMACY AND PHARMACOLOGY, 1998, 50 (10) :1155-1160

[2] Ginsenoside, Re of Panax ginseng possesses significant antioxidant and antihyperlipidemic efficacies in streptozotocin-induced diabetic rats [J].

Cho, William C. S. ;

Chung, Wai-Shing ;

Lee, Sally K. W. ;

Leung, Albert W. N. ;

Cheng, Christopher H. K. ;

Yue, Kevin K. M. .

EUROPEAN JOURNAL OF PHARMACOLOGY, 2006, 550 (1-3) :173-179

[3] Proof of the mysterious efficacy of ginseng: Basic and clinical trials: Metabolic activation of ginsenoside: Deglycosylation by intestinal bacteria and esterification with fatty acid [J].

Hasegawa, H .

JOURNAL OF PHARMACOLOGICAL SCIENCES, 2004, 95 (02) :153-157

[4] Optimization of ginsenosides hydrolyzing β-glucosidase production from Aspergillus niger using response surface methodology [J].

Hu, Jing-Ning ;

Zhu, Xue-Mei ;

Lee, Ki-Teak ;

Zheng, Yi-Nan ;

Li, Wei ;

Han, Li-Kun ;

Fang, Zhe-Ming ;

Gu, Li-Juan ;

Sun, Bai-Sheng ;

Wang, Chun-Yan ;

Sung, Chang-Kuen .

BIOLOGICAL & PHARMACEUTICAL BULLETIN, 2008, 31 (10) :1870-1874

[5] Ginsenoside-Rs4, a new type of ginseng saponin concurrently induces apoptosis and selectively elevates protein levels of p53 and p21WAF1 in human hepatoma SK-HEP-1 cells [J].

Kim, SE ;

Lee, YH ;

Park, JH ;

Lee, SK .

EUROPEAN JOURNAL OF CANCER, 1999, 35 (03) :507-511

[6]

Lee KY, 1997, ANTICANCER RES, V17, P1067

[7] Isolation, synthesis and structures of cytotoxic ginsenoside derivatives [J].

Lei, Jun ;

Li, Xiang ;

Gong, Xiao-Jie ;

Zheng, Yi-Nan .

MOLECULES, 2007, 12 (09) :2140-2150

[8]

LI X, 2000, STUDIES CHEM COMPONE, P95

[9] Isolation and determination of ginsenosides in American ginseng leaves and root extracts by LC-MS [J].

Ligor, T ;

Ludwiczuk, A ;

Wolski, T ;

Buszewski, B .

ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2005, 383 (7-8) :1098-1105

[10] Anti-proliferative effect of ginseng saponins on human prostate cancer cell line [J].

Liu, WK ;

Xu, SX ;

Che, CT .

LIFE SCIENCES, 2000, 67 (11) :1297-1306

← 1 2 →