Polyaniline nanotubes for impedimetric triglyceride detection

被引：48

作者：

Dhand, Chetna ^{[1
,2
]}

Solanki, Pratima. R. ^{[1
]}

Sood, K. N. ^{[1
]}

Datta, Monika ^{[2
]}

Malhotra, B. D. ^{[1
]}

机构：

[1] Natl Phys Lab, Dept Sci & Technol, Ctr Biomol Elect, New Delhi 110012, India

[2] Univ Delhi, Dept Chem, Delhi 110007, India

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2009年 / 11卷 / 07期

关键词：

Polyaniline nanotube; Lipase; Triglyceride; Tributyrin; Impedimetric biosensor; CONDUCTING-POLYMER; SERUM TRIGLYCERIDES; BIOSENSOR; ENZYME; MICRO/NANOTUBES; NANOPARTICLES; SENSORS;

D O I：

10.1016/j.elecom.2009.05.034

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

070208 [无线电物理];

摘要：

Polyaniline nanotubes (PANI-NT) based film electrophoretically deposited onto indium-tin-oxide (ITO) coated glass plate has been utilized for covalent immobilization of lipase (LIP), via glutaraldehyde (Glu), for triglyceride detection using impedimetric technique. It is shown that fatty acid molecules produced during triglyceride hydrolysis result in change in charge transfer resistance (R-CT) of PANI-NT film with varying triglyceride concentration. LIP/Glu/PANI-NT/ITO bioelectrocle has linearity as 25300 mg dL(-1), sensitivity as 2.59 x 10(-3) K Omega(-1) mg(-1) dL, response time as 20 s and regression coefficient as 0.99. A low value of apparent Michaelis-Menten constant (similar to 0.62 mM) indicates high enzyme affinity to tributyrin. The LIP/Glu/PANI-NT/ITO bioelectrode has been utilized to estimate triglyceride in serum samples. (c) 2009 Elsevier B.V. All rights reserved.

引用

页码：1482 / 1486

页数：5

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