Male sterility and enhanced radiation sensitivity in TLS-/- mice

被引：175

作者：

Kuroda, M

Sok, J

Webb, L

Baechtold, H

Urano, F

Yin, Y

Chung, P

de Rooij, DG

Akhmedov, A

Ashley, T

Ron, D ^{[1
]}

机构：

[1] NYU, Med Ctr, Skirball Inst Biomol Med, New York, NY 10016 USA

[2] NYU, Med Ctr, Dept Med, New York, NY 10016 USA

[3] NYU, Med Ctr, Dept Cell Biol, New York, NY 10016 USA

[4] NYU, Med Ctr, Kaplan Canc Ctr, New York, NY 10016 USA

[5] Yale Univ, Sch Med, Dept Genet, New Haven, CT 06510 USA

[6] Basel Inst Immunol, CH-4005 Basel, Switzerland

[7] Univ Utrecht, Sch Med, Dept Cell Biol, NL-3584 CX Utrecht, Netherlands

来源：

EMBO JOURNAL | 2000年 / 19卷 / 03期

关键词：

fusion oncoproteins; homologous recombination; spermatogenesis; TLS deficiency;

D O I：

10.1093/emboj/19.3.453

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

TLS (also known as FUS) is an RNA-binding protein that contributes the N-terminal half of fusion oncoproteins implicated in the development of human liposarcomas and leukemias, Here we report that male mice homozygous for an induced mutation in TLS are sterile with a marked increase in the number of unpaired and mispaired chromosomal axes in premeiotic spermatocytes. Nuclear extracts from TLS-/- testes lack an activity capable of promoting pairing between homologous DNA sequences in vitro, and TLS-/- mice and embryonic fibroblasts exhibit increased sensitivity to ionizing irradiation. These results are consistent with a role for TLS in homologous DNA pairing and recombination.

引用

页码：453 / 462

页数：10

共 43 条

[1] CHARACTERIZATION OF 2 NUCLEAR MAMMALIAN HOMOLOGOUS DNA-PAIRING ACTIVITIES THAT DO NOT REQUIRE ASSOCIATED EXONUCLEASE ACTIVITY [J].