Enantioseparation of a protease inhibitor, indinavir, by subcritical fluid chromatography

被引：21

作者：

Chen, L

Thompson, R

Grinberg, N

Johnson, B

Ellison, D

机构：

[1] Merck Research Laboratories, Rahway, NJ 07065

来源：

JOURNAL OF CHROMATOGRAPHY A | 1996年 / 752卷 / 1-2期

关键词：

enantiomer separation; subcritical fluid chromatography; indinavir;

D O I：

10.1016/S0021-9673(96)00509-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Indinavir is a protease inhibitor which possesses five chiral centers. Enantioseparation of indinavir and its enantiomer was performed on an amylose type stationary phase, Chiralpak(R) AD, under normal-phase HPLC and subcritical fluid chromatography conditions. Under the utilized chromatographic conditions, it is believed that the leading interactions are hydrogen bonding between one or both hydroxyl groups of the solute with the carbonyl group of the carbamate. An inclusion mechanism appears to control the chiral recognition. The effect of various modifiers, pressure, and temperature were investigated.

引用

收藏

页码：101 / 109

页数：9

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