Silver nanoparticles inhibit VEGF induced cell proliferation and migration in bovine retinal endothelial cells

被引：203

作者：

Kalishwaralal, Kalimuthu ^{[1
]}

Banumathi, Elayappan ^{[1
]}

Pandian, SureshBabu Ram Kumar ^{[1
]}

Deepak, Venkataraman ^{[1
]}

Muniyandi, Jeyaraj ^{[1
]}

Eom, Soo Hyun ^{[2
]}

Gurunathan, Sangiliyandi ^{[1
,2
]}

机构：

[1] Kalasalingam Univ, Div Mol & Cellular Biol, Dept Biotechnol, Krishnankoil 626190, Tamil Nadu, India

[2] Gwangju Inst Sci & Technol, Cell Dynam Res Ctr, Dept Life Sci, Kwangju 500712, South Korea

来源：

COLLOIDS AND SURFACES B-BIOINTERFACES | 2009年 / 73卷 / 01期

关键词：

Bacillus licheniformis; Silver nanoparticles; Diabetic retinopathy; Primary bovine retinal endothelial cells; GROWTH-FACTOR; KINASE-B; THERAPY; BIOSYNTHESIS; DELIVERY; PATHWAY; CULTURE;

D O I：

10.1016/j.colsurfb.2009.04.025

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Angiogenesis, the growth of new blood vessels from pre-existing vasculature is of physiological and pathological importance. We have investigated the anti-angiogenic potential of silver nanoparticles, produced by Bacillus licheniformis. Bovine retinal endothelial cells (BRECs) were treated with the different concentrations of silver nanoparticles for 24 h in the presence and absence of vascular endothelial growth factor (VEGF), where 500 nM (IC50) of silver nanoparticle concentration, was able to block the proliferation and migration of BRECs. The cells showed a clear enhancement in caspase-3 activity and formation of DNA ladders, evidence of induction of apoptosis. Here we report for the first time that silver nanoparticles inhibit cell survival via PI3K/Akt dependent pathway in Bovine retinal endothelial cells. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：51 / 57

页数：7

共 35 条

[1] SUPPRESSION OF RETINAL NEOVASCULARIZATION IN-VIVO BY INHIBITION OF VASCULAR ENDOTHELIAL GROWTH-FACTOR (VEGF) USING SOLUBLE VEGF-RECEPTOR CHIMERIC PROTEINS [J].