Sperm protamine 1/protamine 2 ratios are related to in vitro fertilization pregnancy rates and predictive of fertilization ability

被引:136
作者
Aoki, Vincent W.
Liu, Lihua
Jones, Kirtly P.
Hatasaka, Harry H.
Gibson, Mark
Peterson, C. Matthew
Carrell, Douglas T.
机构
[1] Univ Utah, Sch Med, Div Urol, Androl & IVF Labs, Salt Lake City, UT 84108 USA
[2] Univ Utah, Sch Med, Dept Surg, Salt Lake City, UT 84108 USA
[3] Univ Utah, Sch Med, Dept Physiol, Salt Lake City, UT 84108 USA
[4] Univ Utah, Sch Med, Dept Obstet & Gynecol, Salt Lake City, UT 84108 USA
关键词
capacitation; chromatin; fertilization; IVF; protamines;
D O I
10.1016/j.fertnstert.2006.04.024
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Objective: To evaluate whether aberrant sperm P1/P2 ratios are predictive of abnormal fertilizing ability and are related to in vitro fertilization (IVF) outcome. Design: Prospective case-control study. Setting: University-based infertility and IVF clinic. Patient(s): Forty-three male infertility patients with an abnormally reduced P1/P2 ratio, 251 patients with a normal P1/P2 ratio, and 121 patients with an abnormally elevated P1/P2 ratio. Intervention(s): Human IVF, the sperm penetration assay (SPA), and sperm protamine quantification via nuclear protein extraction, gel electrophoresis, and densitometry analysis. Main Outcome Measure(s): Sperm P1/P2 ratios; P1 and P2 quantities; SPA scores; and IVF-fertilization, embryo-quality, pregnancy, delivery, and spontaneous-abortion rates. Result(s): Standard IVF fertilization rates and SPA scores were significantly reduced in patients with abnormally low and high P1/P2 ratios. In vitro fertilization embryo quality was comparable between these groups, but pregnancy rates were significantly reduced in patients with abnormally reduced P1/P2 ratios. Conclusion(s): The P1/P2 ratio has a significant relationship to sperm fertilization ability. The relationship between protamines and fertilization ability is not understood but may be either a reflection of generalized abnormalities during spermiogenesis or an indication of protamine deficiency acting as a regulator or checkpoint of spermatogenesis.
引用
收藏
页码:1408 / 1415
页数:8
相关论文
共 35 条
[1]   DNA integrity is compromised in protamine-deficient human sperm [J].
Aoki, VW ;
Moskovtsev, SI ;
Willis, J ;
Liu, LH ;
Mullen, JBM ;
Carrell, DT .
JOURNAL OF ANDROLOGY, 2005, 26 (06) :741-748
[2]   Identification and evaluation of a novel sperm protamine abnormality in a population of infertile males [J].
Aoki, VW ;
Liu, LH ;
Carrell, DT .
HUMAN REPRODUCTION, 2005, 20 (05) :1298-1306
[3]  
Aoki VW, 2003, ASIAN J ANDROL, V5, P315
[4]   ABERRANT PROTAMINE-1 PROTAMINE-2 RATIOS IN SPERM OF INFERTILE HUMAN MALES [J].
BALHORN, R ;
REED, S ;
TANPHAICHITR, N .
EXPERIENTIA, 1988, 44 (01) :52-55
[5]  
Balhorn R, 1999, MALE GAMETE: FROM BASIC SCIENCE TO CLINICAL APPLICATIONS, P55
[6]  
BARONE JG, 1994, J ANDROL, V15, P139
[7]   HUMAN MALE-INFERTILITY MAY BE DUE TO A DECREASE OF THE PROTAMINE-P2 CONTENT IN SPERM CHROMATIN [J].
BELOKOPYTOVA, IA ;
KOSTYLEVA, EI ;
TOMILIN, AN ;
VOROBEV, VI .
MOLECULAR REPRODUCTION AND DEVELOPMENT, 1993, 34 (01) :53-57
[8]  
CARRELL D, 1996, ASSIST REPROD REV, V6, P170
[9]  
CARRELL DT, 1992, FERTIL STERIL, V57, P134
[10]   A simplified coculture system using homologous, attached cumulus tissue results in improved human embryo morphology and pregnancy rates during in vitro fertilization [J].
Carrell, DT ;
Peterson, CM ;
Jones, KP ;
Hatasaka, HH ;
Udoff, LC ;
Cornwell, CE ;
Thorp, C ;
Kuneck, P ;
Erickson, L ;
Campbell, B .
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 1999, 16 (07) :344-349