Change of Peripheral Blood Mononuclear Cells IFN-γ, IL-10, and TGF-β1 mRNA Expression Levels With Active Human Cytomegalovirus Infection in Orthotopic Liver Transplantation

被引：21

作者：

Zhang, Y.

Wang, Y. -L. ^{[1
,2
,3
]}

Liu, Y. -W. ^{[4
]}

Li, Q.

Yuan, Y. -H.

Niu, W. -Y.

Sun, L. -Y. ^{[2
,3
]}

Zhu, Z. -J. ^{[2
,3
]}

Shen, Z. -Y. ^{[2
,3
]}

Han, R. -F. ^{[1
]}

机构：

[1] Tianjin Med Univ, Tianjin Inst Urol, Tianjin 300211, Peoples R China

[2] Tianjin First Cent Hosp, Orient Organ Transplant Ctr, Tianjin Med Univ Canc Inst & Hosp, Dept Anaesthesia, Tianjin, Peoples R China

[3] Tianjin First Cent Hosp, Orient Organ Transplant Ctr, Tianjin Med Univ Canc Inst & Hosp, Dept Hepatobiliary Surg, Tianjin, Peoples R China

[4] Kuopio Univ Hosp, Dept Clin Radiol, SF-70210 Kuopio, Finland

来源：

TRANSPLANTATION PROCEEDINGS | 2009年 / 41卷 / 05期

关键词：

RISK-FACTOR; RECIPIENTS; INTERLEUKIN-10; REJECTION; PROTEINS; DISEASE; IMPACT;

D O I：

10.1016/j.transproceed.2009.03.064

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Aim. To analyze the expression levels of interferon gamma (IFN-gamma), interleukin 10 (IL-10), and transforming growth factor beta 1 (TGF-beta 1) mRNA in peripheral blood mononuclear cells (PBMCs) in liver transplanted recipients with active HCMV infection. Methods. PBMCs were isolated from 20 liver transplanted recipients with active HCMV infection and 20 recipients without HCMV infection. The expression levels of IFN-gamma, IL-10 and TGF-beta 1 mRNA in PBMCs were measured by TaqMan real-time reverse transcriptase-polymerase chain reaction (RT-PCR). The results were compared with that from 20 healthy individuals. Results. The expression level of TGF-beta 1 mRNA was significantly increased in the active HCMV infection group compared with that in stable group or healthy group (P < .001). The expression level of IL-10 mRNA was significantly increased in the active HCMV infection group compared with the healthy group (P = .001). However, the IFN-gamma mRNA expression level was significantly decreased in the active HCMV infection group compared with that in the stable group or the healthy group (P < .05). Conclusion. Cytokine production plays a role in the HCMV infection. This may provide an important clue to a better understanding of the pathogenesis in liver transplanted recipients with active HCMV infection.

引用

页码：1767 / 1769

页数：3

共 16 条

[1]

Impact of cytomegalovirus infection, year of transplantation, and donor age on outcomes after liver transplantation for hepatitis C [J].