Size-exclusion chromatography of lignin as ion-pair complex

被引：18

作者：

Majcherczyk, A

Huttermann, A

机构：

[1] Institute of Forest Botany, University of Göttingen, 37077 Göttingen

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 764卷 / 02期

关键词：

ion-pairing reagents; lignin; lignosulfonic acids;

D O I：

10.1016/S0021-9673(96)00909-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance ion-pair size-exclusion chromatography method was developed for analysis of underivatised lignin samples using styrene-divinylbenzene gel columns with tetrahydrofuran as solvent. An extraction method using the quaternary amine methyltrioctylammonium chloride allowed quantitative extraction of kraft lignin, lignosulfonic acids and Organosolv lignins from alkaline solution to various organic solvents. Quaternary-amine complexes of lignins formed were analysed by an ion-pair type size-exclusion chromatography in 20 mM quaternary amine in tetrahydrofuran. Intermolecular association and adsorption on the matrix was overcome without sample derivatisation. It was possible to analyse various types of lignins and dehydropolymer-lignin model compounds using the same universal analytical system.

引用

页码：183 / 191

页数：9

共 29 条

[1]

ADLER ERICH, 1958, SVENSK PAPPERSTIDN, V61, P641

[2] SOLUTION PROPERTIES OF LIGNIN . THERMODYNAMIC PROPERTIES AND MOLECULAR WEIGHT DETERMINATIONS [J].