Molecular cloning and expression of CYP6B8:: a xanthotoxin-inducible cytochrome P450 cDNA from Helicoverpa zea

被引:104
作者
Li, XC [1 ]
Berenbaum, MR
Schuler, MA
机构
[1] Nanjing Agr Univ, Dept Plant Protect, Nanjing 210095, Peoples R China
[2] Univ Illinois, Dept Entomol, Urbana, IL 61801 USA
[3] Univ Illinois, Dept Cell & Struct Biol, Urbana, IL 61801 USA
基金
美国国家科学基金会;
关键词
CYP6B8; cytochrome P450; monooxygenase; xanthotoxin; furanocoumarins; alpha-cypermethrin; Helicoverpa zea;
D O I
10.1016/S0965-1748(99)00102-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 [生物化学与分子生物学]; 081704 [应用化学];
摘要
Xanthotoxin, a plant allelochemical, induces a-cypermethrin insecticide tolerance in Helicoverpa tea (corn earworm); inhibition of tolerance by piperonyl butoxide implicates cytochrome P450 monooxygenases (P450s) in the detoxification of this insecticide. To characterize the xanthotoxin-inducible P450 that might mediate a-cypermethrin tolerance in this species, a cDNA library prepared from xanthotoxin-induced H. tea fifth instar larvae was screened with cDNAs encoding furanocoumarin-metabolizing P450s from Papilio polyxenes (CYP6B1 nu 2) and P. glaucus (CYP6B4 nu 2) as well as a sequence-related P450 from Helicoverpa armigera (CYP6B2). One full-length cDNA isolated in this screening shares 51-99% amino acid identity with the CYP6B subfamily of P450s isolated from Papilio and Helicoverpa species and, thus, has been designated CYP6B8. All of these CYP6B subfamily members share a number of highly conserved domains, including substrate recognition site 1 (SRS 1) that is critical for xanthotoxin metabolism by CYP6B1v2 from Papilio polyxenes and coumarin metabolism by CYP2a5 from Mus musculus. Northern and RT-PCR analyses indicate that CYP6B8 expression is strongly induced by xanthotoxin and phenobarbital and negligibly induced by alpha-cypermethrin. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:75 / 84
页数:10
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