Pteridine nucleosides new - New versatile building blocks in oligonucleotide synthesis

被引：12

作者：

Charubala, R ^{[1
]}

Maurinsh, J ^{[1
]}

Rosler, A ^{[1
]}

Melguizo, M ^{[1
]}

Jungmann, O ^{[1
]}

Gottlieb, M ^{[1
]}

Lehbauer, J ^{[1
]}

Hawkins, M ^{[1
]}

Pfleiderer, W ^{[1
]}

机构：

[1] NIH,BETHESDA,MD 20892

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 7-9期

关键词：

D O I：

10.1080/07328319708006188

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chemical syntheses of 1-(2-deoxy-beta-D-ribofuranosyl)lumazines and isopterins as well as 8-(2-deoxy-beta-D-ribofuranosyl)-4-amino-7(8H)pteridones and -isoxanthopterins have been developed to make the structural analogs of the naturally occurring 2'-deoxyribonucleosides in the pteridine series available. The corresponding phosphoramidites have been used in machine-aided solid-support syntheses leading to new types of fluorescence labeled oligonucleotides. The effects of the various fluorophors on duplex formation and as labels for enzyme reactions is demonstrated.

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页码：1369 / 1378

页数：10

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