Optimization of the reaction conditions for the peroxyoxalate chemiluminescence detection system of fluorescent compounds in a high-performance liquid chromatography

被引：9

作者：

Gohda, R ^{[1
]}

Kimoto, K ^{[1
]}

Santa, T ^{[1
]}

Fukushima, T ^{[1
]}

Homma, H ^{[1
]}

Imai, K ^{[1
]}

机构：

[1] UNIV TOKYO,FAC PHARMACEUT SCI,TOKYO 113,JAPAN

来源：

ANALYTICAL SCIENCES | 1996年 / 12卷 / 05期

关键词：

peroxyoxalate chemiluminescence; batch method; high-performance liquid chromatography; bis[4-nitro-2-(3,6,9-trioxadecyloxycarbonyl)phenyl]oxalate; dipyridamole; dansylated valine;

D O I：

10.2116/analsci.12.713

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Using an instrument of automatic fluid injection (batch method), we optimized such reaction conditions as pH, concentration and kinds of buffer for the peroxyoxalate chemiluminscence (PO-CL) detection of fluorescent compounds for high-performance liquid chromatography (HPLC). The results were compared with those obtained by HPLC. Consequently, the usefulness of the batch method for investigating the reaction conditions for PO-CL detection in HPLC was demonstrated. With the optimized condition using a phosphate buffer for the eluent in HPLC, the detection limits for Dns-Val and dipyridamole were 70 amol and 20 amol on column (S/N=2), respectively.

引用

页码：713 / 719

页数：7

共 21 条

[1] CHEMI-LUMINESCENCE FROM REACTIONS OF BIS(PENTACHLOROPHENYL)OXALATE, HYDROGEN-PEROXIDE AND FLUORESCENT COMPOUNDS - KINETICS AND MECHANISM [J].