Gemcitabine transport in Xenopus oocytes expressing recombinant plasma membrane mammalian nucleoside transporters

被引:185
作者
Mackey, JR
Yao, SYM
Smith, KM
Karpinski, E
Baldwin, SA
Cass, CE
Young, JD
机构
[1] Univ Alberta, Dept Oncol, Edmonton, AB T6G 2M7, Canada
[2] Cross Canc Inst, Edmonton, AB T6G 1Z2, Canada
[3] Univ Alberta, Dept Physiol, Edmonton, AB, Canada
[4] Univ Leeds, Sch Biochem & Mol Biol, Leeds, W Yorkshire, England
来源
JNCI-JOURNAL OF THE NATIONAL CANCER INSTITUTE | 1999年 / 91卷 / 21期
关键词
D O I
10.1093/jnci/91.21.1876
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Background: Gemcitabine, a pyrimidine analogue of deoxycytidine, is an anticancer nucleoside drug that requires functional plasma membrane nucleoside transporter proteins to reach its intracellular targets and cause cytotoxicity, Because of technical difficulties inherent in studying nucleoside transport in human cells, we rigorously defined gemcitabine membrane transportability by producing each of the available human (h) and rat (r) recombinant nucleoside transporters (NTs) individually in Xenopus laevis oocytes, Methods: Oocytes were microinjected with in vitro-transcribed RNAs derived from complementary DNAs encoding (C = concentrative) rCNT1, rCNT2, hCNT1, hCNT2, (E = equilibrative) rENT1, rENT2, hENT1, and hENT2, Uptake of [H-3]gemcitabine and [C-14] uridine was measured 3 days after microinjection to determine kinetic constants. We also used the two-electrode, voltage-clamp technique to investigate the electrophysiology of hCNT1-mediated gemcitabine transport. Results: Gemcitabine was transported by most of the tested proteins (the exceptions being the purine-selective rCNT2 and hCNT2), with the greatest uptake occurring in oocytes producing recombinant rCNT1 and hCNT1, Influxes of gemcitabine mediated by hCNT1, hENT1, and hENT2 were saturable and conformed to Michaelis-Menten kinetics with apparent K-m values of 24, 160, and 740 mu M, respectively. Gemcitabine had a limited ability to cross the lipid bilayer of oocyte membranes by simple diffusion. External application of gemcitabine to oocytes producing recombinant hCNT1 induced an inward current, which demonstrated that hCNT1 functions as a Na+/nucleoside co-transport protein and confirmed the transporter's ability to transport gemcitabine, Conclusions: Mammalian nucleoside transporters vary widely in their affinity and capacity to transport gemcitabine. Variation in the tumor and tissue distribution of plasma membrane nucleoside transporter proteins may contribute to the solid tumor activities and schedule-dependent toxic effects of gemcitabine.
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页码:1876 / 1881
页数:6
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