Biological ingredient analysis of traditional Chinese medicine preparation based on high-throughput sequencing: the story for Liuwei Dihuang Wan

被引：115

作者：

Cheng, Xinwei ^{[1
,3
]}

Su, Xiaoquan ^{[2
]}

Chen, Xiaohua ^{[2
]}

Zhao, Huanxin ^{[1
]}

Bo, Cunpei ^{[2
]}

Xu, Jian ^{[2
]}

Bai, Hong ^{[1
]}

Ning, Kang ^{[2
]}

机构：

[1] Shandong Acad Med Sci, Inst Mat Med, Jinan 250062, Shandong, Peoples R China

[2] Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels & Bioinformat, Grp SingleCell Ctr,Shandong Key Lab Energy Genet, Qingdao 266101, Peoples R China

[3] Univ Jinan, Shandong Acad Med Sci, Sch Med & Life Sci, Jinan 250062, Peoples R China

来源：

SCIENTIFIC REPORTS | 2014年 / 4卷

基金：

中国国家自然科学基金;

关键词：

QUALITY-CONTROL; DNA BARCODES; HERBAL DRUGS; IDENTIFICATION; AUTHENTICATION; SYSTEM; PLANTS;

D O I：

10.1038/srep05147

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Although Traditional Chinese Medicine (TCM) preparations have long history with successful applications, the scientific and systematic quality assessment of TCM preparations mainly focuses on chemical constituents and is far from comprehensive. There are currently only few primitive studies on assessment of biological ingredients in TCM preparations. Here, we have proposed a method, M-TCM, for biological assessment of the quality of TCM preparations based on high-throughput sequencing and metagenomic analysis. We have tested this method on Liuwei Dihuang Wan (LDW), a TCM whose ingredients have been well-defined. Our results have shown that firstly, this method could determine the biological ingredients of LDW preparations. Secondly, the quality and stability of LDW varies significantly among different manufacturers. Thirdly, the overall quality of LDW samples is significantly affected by their biological contaminations. This novel strategy has the potential to achieve comprehensive ingredient profiling of TCM preparations.

引用

页数：12

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