Influence of dietary phytosterols and phytostanols on diastolic blood pressure and the expression of blood pressure regulatory genes in SHRSP and WKY inbred rats

被引：17

作者：

Chen, Qixuan ^{[1
]}

Gruber, Heidi ^{[1
]}

Swist, Eleonora ^{[1
]}

Pakenham, Catherine ^{[1
]}

Ratnayake, Walisundera M. N. ^{[1
]}

Scoggan, Kylie A. ^{[1
,2
]}

机构：

[1] Hlth Canada, Food Directorate, Hlth Prod & Food Branch, Nutr Res Div,Banting Res Ctr, Ottawa, ON K1A 0K9, Canada

[2] Univ Ottawa, Dept Biochem Microbiol & Immunol, Ottawa, ON K1H 8M5, Canada

来源：

BRITISH JOURNAL OF NUTRITION | 2009年 / 102卷 / 01期

关键词：

Phytosterols; Phytostanols; Blood pressure; Gene expression; Rats; RENIN-ANGIOTENSIN SYSTEM; SERUM PLANT STEROLS; NITRIC-OXIDE; RAPESEED OIL; ENDOTHELIAL DYSFUNCTION; OXIDATIVE STRESS; SOYBEAN OIL; LIFE-SPAN; SUPEROXIDE; CHOLESTEROL;

D O I：

10.1017/S0007114508137904

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 [营养与食品卫生学];

摘要：

The aim of the present study was to determine the impact of increased consumption of phytosterols or phytostanols on blood pressure and renal blood pressure regulatory gene expression in stroke-prone spontaneously hypertensive (SHRSP) and normotensive Wistar-Kyoto (WKY) inbred rats. SHRSP and WKY inbred rats (10/group) were fed a control diet or a diet supplemented with phytosterols or phytostanols (2.0 g/kg diet). After 5 weeks, SHRSP rats demonstrated higher systolic and diastolic blood pressures than WKY inbred rats. SHRSP rats that consumed the phytosterol or phytostanol supplemental diets displayed a 2- or 3-fold respective increase in the diastolic blood pressure than those that consumed the control diet. Angiotensinogen (Agt), angiotensin I-converting enzyme I (Ace1), nitric oxide synthase (Nos) 1, Nos3, cyclooxygenase 2 (Cox2) and THUMP domain containing I were expressed at higher levels in SHRSP compared with WKY inbred rats. Renin and angiotensin II receptor type la were expressed at lower levels in SHRSP than WKY inbred rats. Phytostanol supplementation up-regulated the expression of Ace1 and Nos3 in SHRSP rats. Phytosterol supplementation increased the mRNA levels of Nos1 and spondin I (Spon1) in SHRSP and WKY inbred rats. Cox2 mRNA levels were elevated in both phytosterol- and phytostanol-supplemented SHRSP and WKY inbred rats. Therefore, the increased blood pressure in SHRSP rats may be partly due to altered renal expression of blood pressure regulatory genes. Specifically, up-regulation of Ace1, Nos1, Nos3, Cox2 and Spon1 were associated with the increased diastolic blood pressure observed in phytosterol- or phytostanol-supplemented SHRSP rats.

引用

页码：93 / 101

页数：9

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