Proenkephalin A-derived peptides in invertebrate innate immune processes

被引:48
作者
Tasiemski, A
Verger-Bocquet, M
Cadet, M
Goumon, Y
Metz-Boutigue, MH
Aunis, D
Stefano, GB
Salzet, M
机构
[1] Univ Sci & Technol Lille, CNRS, UPRES A 8017, Lab Endocrinol Annelides, F-59655 Villeneuve Dascq, France
[2] SUNY Coll Old Westbury, Multidisciplinary Ctr Study Aging, Neurosci Res Inst, Old Westbury, NY 11568 USA
[3] INSERM, U338 Biol Commun Cellulaire, F-67084 Strasbourg, France
来源
MOLECULAR BRAIN RESEARCH | 2000年 / 76卷 / 02期
关键词
antibacterial peptide; opioid; innate immunity; neuroimmunity;
D O I
10.1016/S0169-328X(00)00005-X
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Lipopolysaccharides (LPS) injection into the coelomic fluid of the leech Theromyzon tessulatum stimulates release of proenkephalin A (PEA)-derived peptides as determined by immunoprecipitation and Western blot analyses. This release occurs in the first 15 min after LPS exposure and yields a 5.3-kDa peptide fragment corresponding to the C-terminal part of the precursor. This fragment is then cleaved to free an antibacterial peptide related to mammals arginine phenylalanine extended enkelytin: the peptide B. These PEA processing peptides were characterized using a combination of techniques including reversed-phase HPLC, microsequencing and mass spectrometry. The isolated invertebrate peptide B presents a high sequence homology with the bovine's and the same activity against Gram + bacteria. Titrations revealed the simultaneous appearance of Methionine-enkephalin (ME) and peptide B in invertebrates after stimulation by LPS (in a dose-dependent manner), surgical trauma or electrical stimulations to neural tissues of the mussel. Furthermore, peptide B processing in vitro yields Methionine-enkephalin arginine phenylalanine (MERF), which exhibits via the delta receptors, immunocyte excitatory properties, i.e., movement and conformational changes, but no antibacterial activity. We surmise that this unified response to the various stimuli is a survival strategy for organism by providing immediate antibacterial activity and immunocyte stimulation, thereby reducing any immune latency period needed for an adequate immune response. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:237 / 252
页数:16
相关论文
共 38 条
[1]  
BRESLIN MB, 1993, J BIOL CHEM, V268, P27084
[2]   Drosophila antimicrobial peptides [J].
Bulet, P .
M S-MEDECINE SCIENCES, 1999, 15 (01) :23-29
[3]  
BULET P, 1993, J BIOL CHEM, V268, P14893
[4]   The C-terminal bisphosphorylated proenkephalin-A-(209-237)-peptide from adrenal medullary chromaffin granules possesses antibacterial activity [J].
Goumon, Y ;
Strub, JM ;
Moniatte, M ;
Nullans, G ;
Poteur, L ;
Hubert, P ;
VanDorsselaer, A ;
Aunis, D ;
MetzBoutigue, MH .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 235 (03) :516-525
[5]   Characterization of antibacterial COOH-terminal proenkephalin-A-derived peptides (PEAP) in infectious fluids -: Importance of enkelytin, the antibacterial PEAP209-237 secreted by stimulated chromaffin cells [J].
Goumon, Y ;
Lugardon, K ;
Kieffer, B ;
Lefèvre, JF ;
Van Dorsselaer, A ;
Aunis, D ;
Metz-Boutigue, MH .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (45) :29847-29856
[6]   Structure-function relationships of antimicrobial peptides [J].
Hwang, PM ;
Vogel, HJ .
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1998, 76 (2-3) :235-246
[7]   A novel peptide-processing activity of insect peptidyl-dipeptidase A (angiotensin I-converting enzyme): the hydrolysis of lysyl-arginine and arginyl-arginine from the C-terminus of an insect prohormone peptide [J].
Isaac, RE ;
Schoofs, L ;
Williams, TA ;
Veelaert, D ;
Sajid, M ;
Corvol, P ;
Coates, D .
BIOCHEMICAL JOURNAL, 1998, 330 :61-65
[8]   Specificity of prohormone convertase 2 on proenkephalin and proenkephalin-related substrates [J].
Johanning, K ;
Juliano, MA ;
Juliano, L ;
Lazure, C ;
Lamango, NS ;
Steiner, DF ;
Lindberg, I .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (35) :22672-22680
[9]   T helper 2 cytokines induce preproenkephalin mRNA expression and proenkephalin A in human peripheral blood mononuclear cells [J].
Kamphuis, S ;
Kavelaars, A ;
Brooimans, R ;
Kuis, W ;
Zegers, BJM ;
Heijnen, CJ .
JOURNAL OF NEUROIMMUNOLOGY, 1997, 79 (01) :91-99
[10]   Role of endogenous pro-enkephalin A-derived peptides in human T cell proliferation and monocyte IL-6 production [J].
Kamphuis, S ;
Eriksson, F ;
Kavelaars, A ;
Zijlstra, J ;
van de Pol, M ;
Kuis, W ;
Heijnen, CJ .
JOURNAL OF NEUROIMMUNOLOGY, 1998, 84 (01) :53-60