New Amniotic Membrane Based Biocomposite for Future Application in Reconstructive Urology

被引：57

作者：

Adamowicz, Jan ^{[1
,2
]}

Pokrywczynska, Marta ^{[1
]}

Tworkiewicz, Jakub ^{[1
,3
]}

Kowalczyk, Tomasz ^{[4
]}

van Breda, Shane V. ^{[5
]}

Tyloch, Dominik ^{[1
,2
]}

Kloskowski, Tomasz ^{[1
]}

Bodnar, Magda ^{[6
]}

Skopinska-Wisniewska, Joanna ^{[7
]}

Marszalek, Andrzej ^{[6
]}

Frontczak-Baniewicz, Malgorzata ^{[8
]}

Kowalewski, Tomasz A. ^{[9
]}

Drewa, Tomasz ^{[1
,2
,3
]}

机构：

[1] Nicolaus Copernicus Univ Torun, Ludwik Rydygier Med Coll Bydgoszcz, Dept Regenerat Med, Chair Urol, Bydgoszcz, Poland

[2] Nicolaus Copernicus Univ, Dept Gen Oncol & Pediat Urol, Bydgoszcz, Poland

[3] Nicolaus Copernicus Hosp Batory, Dept Urol, Torun, Poland

[4] Polish Acad Sci, Inst Fundamental Technol Res, Lab Modeling Biol & Med, Warsaw, Poland

[5] Univ Pretoria, Dept Internal Med, Div Infect Dis, ZA-0002 Pretoria, South Africa

[6] Nicolaus Copernicus Univ, Fac Med, Dept Clin Pathomorphol, Bydgoszcz, Poland

[7] Nicolaus Copernicus Univ, Fac Chem, Dept Chem Biomat & Cosmet, Bydgoszcz, Poland

[8] Polish Acad Sci, Mossakowski Med Res Ctr, Electron Microscopy Platform, Warsaw, Poland

[9] Polish Acad Sci, Inst Fundamental Technol Res, Dept Mech & Phys Fluids, Warsaw, Poland

来源：

PLOS ONE | 2016年 / 11卷 / 01期

关键词：

SMOOTH-MUSCLE; URINARY-BLADDER; BUCCAL MUCOSA; CROSS-LINKING; FETAL WOUNDS; STEM-CELLS; REGENERATION; TRANSPLANTATION; MATRIX; GRAFT;

D O I：

10.1371/journal.pone.0146012

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Objective Due to the capacity of the amniotic membrane (Am) to support re-epithelisation and inhibit scar formation, Am has a potential to become a considerable asset for reconstructive urology i.e., reconstruction of ureters and urethrae. The application of Am in reconstructive urology is limited due to a poor mechanical characteristic. Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance, without affecting its unique bioactivity profile. This study evaluated biocomposite material composed of Am and nanofibers as a graft for urinary bladder augmentation in a rat model. Material and Methods Sandwich-structured biocomposite material was constructed from frozen Am and covered on both sides with two-layered membranes prepared from electrospun poly-(L-lactide-co-E-caprolactone) (PLCL). Wistar rats underwent hemicystectomy and bladder augmentation with the biocomposite material. Results Immunohistohemical analysis (hematoxylin and eosin [H&E], anti-smoothelin and Masson's trichrome staining [TRI]) revealed effective regeneration of the urothelial and smooth muscle layers. Anti-smoothelin staining confirmed the presence of contractile smooth muscle within a new bladder wall. Sandwich-structured biocomposite graft material was designed to regenerate the urinary bladder wall, fulfilling the requirements for normal bladder tension, contraction, elasticity and compliance. Mechanical evaluation of regenerated bladder wall conducted based on Young's elastic modulus reflected changes in the histological remodeling of the augmented part of the bladder. The structure of the biocomposite material made it possible to deliver an intact Am to the area for regeneration. An unmodified Am surface supported regeneration of the urinary bladder wall and the PLCL membranes did not disturb the regeneration process. Conclusions Am reinforcement with electrospun nanofibers offers a new strategy to improve Am mechanical resistance without affecting its unique bioactivity profile.

引用

页数：20

共 84 条

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