Activity, tissue distribution and site-directed mutagenesis of a human peptide methionine sulfoxide reductase of type B: hCBS1

被引：61

作者：

Jung, S

Hansel, A

Kasperczyk, H

Hoshi, T

Heinemann, SH

机构：

[1] Univ Jena, Fac Med, D-07747 Jena, Germany

[2] Univ Penn, Dept Physiol, Philadelphia, PA 19104 USA

来源：

FEBS LETTERS | 2002年 / 527卷 / 1-3期

关键词：

methionine sulfoxide; reactive oxygen species; oxidative stress; posttranslational modification; potassium channel;

D O I：

10.1016/S0014-5793(02)03171-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human CBS1 is a methionine sulfoxide reductase of type B (MSRB) as it specifically reduced Met-R-SO in peptides with dithiothreitol or the thioredoxin system as reductants. Mutation C169S in the active site completely abolished enzymatic activity, while mutation W110A only reduced activity and C105S had no effect. Like human MSRA, hCBS1 showed in vivo reducing activity coexpressed with the Drosophila ShC/B potassium channel in oocytes, by accelerating the overall inactivation time course. hCBS1-encoding mRNA is most abundant in muscle tissues, especially in the heart and thereby shows an expression pattern different to the human MSRA. (C) 2002 Federation of European Biochemical Societies.

引用

收藏

页码：91 / 94

页数：4

相关论文

共 18 条

[1] Mammalian selenium-containing proteins [J].

Behne, D ;

Kyriakopoulos, A .

ANNUAL REVIEW OF NUTRITION, 2001, 21 :453-473

[2] Modulation of potassium channel function by methionine oxidation and reduction [J].

Ciorba, MA ;

Heinemann, SH ;

Weissbach, H ;

Brot, N ;

Hoshi, T .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (18) :9932-9937

[3] Repair of oxidized proteins - Identification of a new methionine sulfoxide reductase [J].

Grimaud, R ;

Ezraty, B ;

Mitchell, JK ;

Lafitte, D ;

Briand, C ;

Derrick, PJ ;

Barras, F .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (52) :48915-48920

[4] Mitochondrial targeting of the human peptide methionine sulfoxide reductase (MSRA), an enzyme-involved in the repair of oxidized proteins [J].

Hansel, A ;

Kuschel, L ;

Hehl, S ;

Lemke, C ;

Agricola, HJ ;

Hoshi, T ;

Heinemann, SH .

FASEB JOURNAL, 2002, 16 (06) :911-+

[5] Regulation of cell function by methionine oxidation and reduction [J].

Hoshi, T ;

Heinemann, SH .

JOURNAL OF PHYSIOLOGY-LONDON, 2001, 531 (01) :1-11

[6] Identification, expression and chromosome localization of a human gene encoding a novel protein with similarity to the pilB family of transcriptional factors (pilin) and to bacterial peptide methionine sulfoxide reductases [J].

Huang, WH ;

Escribano, J ;

Sarfarazi, M ;

Coca-Prados, M .

GENE, 1999, 233 (1-2) :233-240

[7] Molecular cloning and functional expression of a human peptide methionine sulfoxide reductase (hMsrA) [J].

Kuschel, L ;

Hansel, A ;

Schöherr, R ;

Weissbach, H ;

Brot, N ;

Hoshi, T ;

Heinemann, SH .

FEBS LETTERS, 1999, 456 (01) :17-21

[8] Novel selenoproteins identified in silico and in vivo by using a conserved RNA structural motif [J].

Lescure, A ;

Gautheret, D ;

Carbon, P ;

Krol, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (53) :38147-38154

[9] Structure and mechanism of peptide methionine sulfoxide reductase, an "anti-oxidation" enzyme [J].

Lowther, WT ;

Brot, N ;

Weissbach, H ;

Matthews, BW .

BIOCHEMISTRY, 2000, 39 (44) :13307-13312

[10] The mirrored methionine sulfoxide reductases of Neisseria gonorrhoeae pilB [J].

Lowther, WT ;

Weissbach, H ;

Etienne, F ;

Brot, N ;

Matthews, BW .

NATURE STRUCTURAL BIOLOGY, 2002, 9 (05) :348-352