An oral microjet vaccination system elicits antibody production in rabbits

被引:58
作者
Aran, Kiana [1 ,2 ,3 ,4 ]
Chooljian, Marc [1 ,3 ,5 ,6 ]
Paredes, Jacobo [1 ,3 ,7 ]
Rafi, Mohammad [1 ]
Lee, Kunwoo [1 ,5 ,6 ]
Kim, Allison Y. [1 ]
An, Jeanny [1 ]
Yau, Jennifer F. [1 ]
Chum, Helen [8 ]
Conboy, Irina [1 ,4 ,5 ,6 ]
Murthy, Niren [1 ,3 ,5 ,6 ]
Liepmann, Dorian [1 ,3 ,5 ,6 ]
机构
[1] Univ Calif UC Berkeley, Dept Bioengn, Berkeley, CA 94720 USA
[2] Keck Grad Inst, Sch Appl Life Sci, Claremont, CA 91711 USA
[3] Berkeley Sensor & Actuator Ctr, Berkeley, CA 94720 USA
[4] Buck Inst Res Aging, Novato, CA 94945 USA
[5] UC Berkeley UC San Francisco, Grad Program Bioengn, Berkeley, CA 94158 USA
[6] UC Berkeley UC San Francisco, Grad Program Bioengn, San Francisco, CA 94158 USA
[7] Univ Navarra, Technol Campus, Ctr Studies & Tech Res Gipuzkoa & Tecnun, San Sebastian 20018, Spain
[8] Univ Calif Berkeley, Off Lab Anim Care, Berkeley, CA 94720 USA
关键词
DENDRITIC CELLS; MUCOSAL VACCINES; T-CELLS; OVALBUMIN; IMMUNIZATION; INDUCTION; ADJUVANTS; RESPONSES; DELIVERY; IMMUNITY;
D O I
10.1126/scitranslmed.aaf6413
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
Noninvasive immunization technologies have the potential to revolutionize global health by providing easy-to-administer vaccines at low cost, enabling mass immunizations during pandemics. Existing technologies such as transdermal microneedles are costly, deliver drugs slowly, and cannot generate mucosal immunity, which is important for optimal immunity against pathogens. We present a needle-free microjet immunization device termedMucoJet, which is a three-dimensional microelectromechanical systems-based drug delivery technology. MucoJet is administered orally, placed adjacent to the buccal tissue within the oral cavity, and uses a self-contained gas-generating chemical reaction within its twocompartment plastic housing to produce a high-pressure liquid jet of vaccine. We show that the vaccine jet ejected from the MucoJet device is capable of penetrating the buccal mucosal layer in silico, in porcine buccal tissue ex vivo, and in rabbits in vivo. Rabbits treated with ovalbumin by MucoJet delivery have antibody titers of anti-ovalbumin immunoglobulins G and A in blood serum and buccal tissue, respectively, that are three orders of magnitude higher than rabbits receiving free ovalbumin delivered topically by a dropper in the buccal region. MucoJet has the potential to accelerate the development of noninvasive oral vaccines, given its ability to elicit antibody production that is detectable locally in the buccal tissue and systemically via the circulation.
引用
收藏
页数:9
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