Tougu Xiaotong capsule promotes chondrocyte autophagy by regulating the Atg12/LC3 conjugation systems

被引：24

作者：

Li, Xihai ^{[1
]}

Liu, Fayuan ^{[1
]}

Liang, Wenna ^{[2
]}

Ye, Hongzhi ^{[1
]}

Li, Huiting ^{[3
]}

Yu, Fangrong ^{[3
]}

Chen, Jiashou ^{[3
]}

Chen, Wenlie ^{[1
]}

Lin, Ruhui ^{[3
]}

Zheng, Chunsong ^{[1
]}

Wu, Guangwen ^{[1
]}

Xu, Huifeng ^{[1
]}

Liu, Xianxiang ^{[1
]}

机构：

[1] Fujian Univ Tradit Chinese Med, Acad Integrat Med, Fuzhou 350122, Fujian, Peoples R China

[2] Fujian Univ Tradit Chinese Med, Res Base Tradit Chinese Med Syndrome, Fuzhou 350122, Fujian, Peoples R China

[3] Fujian Univ Tradit Chinese Med, Fujian Key Lab Integrat Med Geriatr, Fuzhou 350122, Fujian, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE | 2014年 / 34卷 / 02期

基金：

中国国家自然科学基金;

关键词：

autophagy; chondrocyte; Tougu Xiaotong capsule; osteoarthritis; AGING-RELATED LOSS; ARTICULAR-CARTILAGE; CELL-DEATH; OSTEOARTHRITIS; APOPTOSIS; DISEASE; DEGRADATION; SUPPRESSION; LUMIRACOXIB; ACTIVATION;

D O I：

10.3892/ijmm.2014.1794

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

100103 [病原生物学]; 100218 [急诊医学];

摘要：

We have previously reported that Tougu Xiaotong capsule (TXC) inhibits tidemark replication and cartilage degradation by regulating chondrocyte autophagy in vivo. Autophagy, a cell protective mechanism for maintaining cellular homeostasis, has been shown to be a constitutively active and protective process for chondrocyte survival. However, it remains unclear whether TXC promotes chondrocyte autophagy by regulating the autophagy-related (Atg)12/microtubule-associated protein 1 light chain 3 (LC3) conjugation systems. Thus, in the present study, we investigated the effects of TXC on primary chondrocytes treated with cobalt chloride (CoCl2). We found that CoCl2, induced a decrease in chondrocyte viability and the autophagosome formation of chondrocytes, indicating that CoCl2, induced autophagic death in a dose- and time-dependent manner. To determine the effects of TXC on CoCl2-exposed chondrocytes, we assessed cell viability by MTT assay. Our results revealed that TXC enhanced the viability of CoCl2-exposed chondrocytes. To gain insight into the mechanisms responsible for the enhancing effects of TXC on CoCl2-exposed chondrocytes, the expression of Atg genes was assessed in chondrocytes exposed to CoCl2 and treated with or without TXC. The results revealed that the expression of beclin 1, Atg3, Atg5, Atg7, Atg10, Atg12 and LC3 II/LC3 I in the chondrocytes treated with TXC increased, compared to that in the untreated chondrocytes. In addition, ultrastructural analysis indicated that treated chondrocytes contained more autophagosomes than the untreated cells, suggesting that TXC increased the formation of autophagosomes in the chondrocytes to clear the CoCl2-induced autophagic death. Therefore, these data suggest that TXC is a potential therapeutic agent for the reduction of cartilage degradation that occurs in osteoarthritis.

引用

页码：545 / 552

页数：8

共 36 条

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