Vrn-D4 is a vernalization gene located on the centromeric region of chromosome 5D in hexaploid wheat

被引：97

作者：

Yoshida, Tetsuya ^{[1
]}

Nishida, Hidetaka ^{[1
]}

Zhu, Jie ^{[2
,3
]}

Nitcher, Rebecca ^{[3
]}

Distelfeld, Assaf ^{[3
]}

Akashi, Yukari ^{[1
]}

Kato, Kenji ^{[1
]}

Dubcovsky, Jorge ^{[3
]}

机构：

[1] Okayama Univ, Grad Sch Nat Sci & Technol, Kita Ku, Okayama 7008530, Japan

[2] China Agr Univ, Dept Genet & Plant Breeding, Beijing 100193, Peoples R China

[3] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

来源：

THEORETICAL AND APPLIED GENETICS | 2010年 / 120卷 / 03期

基金：

美国农业部; 日本学术振兴会;

关键词：

QUANTITATIVE TRAIT LOCI; GROWTH HABIT; MOLECULAR CHARACTERIZATION; ALLELIC VARIATION; GEOGRAPHICAL VARIATION; GLOBAL IMPROVEMENT; COMMON WHEAT; PROTEIN; DIFFERENTIATION; BARLEY;

D O I：

10.1007/s00122-009-1174-3

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Natural variation in wheat requirement of long exposures to cold temperatures to accelerate flowering (vernalization) is mainly controlled by the Vrn-1, Vrn-2, Vrn-3, and Vrn-4 loci. The first three loci have been well characterized, but limited information is available for Vrn-4. So far, natural variation for Vrn-4 has been detected only in the D genome (Vrn-D4), and genetic stocks for this gene are available in Triple Dirk (TDF, hereafter). We detected heterogeneity in the Vrn-1 alleles present in different TDF stocks, which may explain inconsistencies among previous studies. A correct TDF seed stock from Japan carrying recessive vrn-A1, vrn-B1, and vrn-D1 alleles was crossed with three different winter cultivars to generate F-2 mapping populations. Most of the variation in flowering time in these three populations was controlled by a single locus, Vrn-D4, which was mapped within a 1.8 cM interval flanked by markers Xcfd78 and Xbarc205 in the centromeric region of chromosome 5D. A factorial ANOVA for heading time using Vrn-D4 alleles and vernalization as factors showed a significant interaction (P < 0.0001), which confirmed that the Vrn-D4 effect on flowering time is modulated by vernalization. Comparison of the different Triple Dirk stocks revealed that Vrn-B1, Vrn-D1, and Vrn-D4 all have a small residual response to vernalization, but Vrn-D4 differs from the other two in its response to short vernalization periods. The precise mapping and characterization of Vrn-D4 presented here represent a first step toward the positional cloning of this gene.

引用

页码：543 / 552

页数：10

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