Microsatellites in Malus X domestica (apple): Abundance, polymorphism and cultivar identification

被引：521

作者：

Guilford, P ^{[1
]}

Prakash, S ^{[1
]}

Zhu, JM ^{[1
]}

Rikkerink, E ^{[1
]}

Gardiner, S ^{[1
]}

Bassett, H ^{[1
]}

Forster, R ^{[1
]}

机构：

[1] BACHELAR RES CTR,PALMERSTON NORTH,NEW ZEALAND

来源：

THEORETICAL AND APPLIED GENETICS | 1997年 / 94卷 / 02期

关键词：

microsatellites; apple; identification;

D O I：

10.1007/s001220050407

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 [作物学];

摘要：

Screening of an apple genomic library with (GA)15 and (GT)15 probes demonstrated that these repeats are abundant, occurring about every 120 and 190 kb, respectively. Microsatellites isolated from a small insert library enriched for (GA) repeats contained numbers of repeats ranging from 7 to 39. Primers to these microsatellite loci were able to direct the amplification of the repeats in 21 different cultivars. The majority of markers were highly polymorphic, diploid, and showed simple Mendelian inheritance, although about 25% of markers generated complex banding patterns consistent with the amplification of more than one locus. As few as three microsatellite markers were sufficient to differentiate between all 21 cultivars.

引用

页码：249 / 254

页数：6

共 21 条

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