Micro- and macrochromosome paints generated by flow cytometry and microdissection: tools for mapping the chicken genome

被引：92

作者：

Griffin, DK ^{[1
]}

Haberman, F

Masabanda, J

O'Brien, P

Bagga, M

Sazanov, A

Smith, J

Burt, DW

Ferguson-Smith, M

Wienberg, J

机构：

[1] Brunel Univ, Dept Biol Sci, Uxbridge UB8 3PH, Middx, England

[2] Univ Cambridge, Dept Pathol, Cambridge CB2 1QP, England

[3] Univ Munich, Inst Anthropol & Humangenet, Munich, Germany

[4] Roslin Inst, Roslin EH25 9PS, Midlothian, Scotland

[5] Tech Univ Munich, Lehrstuhl Tierzucht, D-8050 Freising, Weihenstephan, Germany

来源：

CYTOGENETICS AND CELL GENETICS | 1999年 / 87卷 / 3-4期

关键词：

D O I：

10.1159/000015449

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Despite the chicken being one of the most genetically mapped of all animals, its karyotype remains poorly defined. This is primarily due to microchromosomes that belie assignment by conventional methods. To address this problem, we have developed chromosome-specific paints using flow cytometry and microdissection. For the microchromosomes it was necessary to amplify and label DNA from single microdissected chromosomes. Copyright (C) 2000 S. Karger AG, Basel.

引用

页码：278 / 281

页数：4

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