Sendai viral vector mediated angiopoietin-1 gene transfer for experimental ischemic limb disease

被引：51

作者：

Huang, Jianhua ^{[1
,2
]}

Inoue, Makoto ^{[3
]}

Hasegawa, Mamoru ^{[3
]}

Tomihara, Kei ^{[1
]}

Tanaka, Toshihiro ^{[1
]}

Chen, Jiahua ^{[2
]}

Hamada, Hirofumi ^{[1
]}

机构：

[1] Sapporo Med Univ, Dept Mol Med, Chuo Ku, Sapporo, Hokkaido 0608556, Japan

[2] NingXia Peoples Hosp, Dept Thorac & Cardiovasc Surg, Yinchuan 750021, Peoples R China

[3] DNAVEC Res Corp, Tsukuba, Ibaraki, Japan

来源：

ANGIOGENESIS | 2009年 / 12卷 / 03期

关键词：

Peripheral arterial obstructive disease; Sendai virus vector; Angiopoietin-1; Ischemia; Lower limb; Rat; ENDOTHELIAL GROWTH-FACTOR; VIRUS VECTORS; AUGMENTS REVASCULARIZATION; INTRAMUSCULAR INJECTION; CELL-SURVIVAL; TIE2; RECEPTOR; EXPRESSION; RNA; ANGIOGENESIS; VEGF;

D O I：

10.1007/s10456-009-9144-6

中图分类号：

R6 [外科学];

学科分类号：

100210 [外科学];

摘要：

Sendai virus vector is emerging as a promising vector for gene therapy, and angiopoietin-1 (Ang-1) has been reported to improve the blood flow recovery in the ischemic limb or heart. In this study, we constructed a human Ang-1-expressing Sendai viral vector (SeVhAng-1) and injected it into the ischemic limb of rats. We found that SeVhAng-1 improved the blood flow recovery and increased the capillary density of the ischemic limb, compared with the controls. We also found that SeVhAng-1 increased p-Akt during the early period of limb ischemia, and decreased apoptosis in ischemic limb. It suggests that SeVhAng-1 may serve as a potential therapeutic tool in ischemic limb disease.

引用

页码：243 / 249

页数：7

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