Quorum sensing inhibition activity of garlic extract and p-coumaric acid

被引：102

作者：

Bodini, S. F. ^{[1
]}

Manfredini, S. ^{[1
]}

Epp, M. ^{[1
]}

Valentini, S. ^{[1
]}

Santori, F. ^{[1
]}

机构：

[1] ISRIM Scarl, Dept Environm Biotechnol, I-05100 Terni, Italy

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2009年 / 49卷 / 05期

关键词：

Agrobacterium tumefaciens; Chromobacterium violaceum; DMSO; garlic; LuxR-type receptors; p-coumaric acid; Pseudomonas chlororaphis; quorum sensing; PSEUDOMONAS; CONSTRUCTION; MOLECULES;

D O I：

10.1111/j.1472-765X.2009.02704.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Aims: The goal of this work was to investigate the influence of DMSO, garlic extract and p-coumaric acid on bacterial quorum sensing (QS). Methods and Results: The decreases in the QS responses of QS reporter strains Escherichia coli pSB401 and pSB536, Agrobacterium tumefaciens NTL4, Chromobacterium violaceum 5999 and wt 494, Pseudomonas putida IsoF/gfp and environmental Pseudomonas chlororaphis were quantified in relation to growth inhibitory effects. DMSO showed no significant QS-specific effects on the strains tested even at close-to-lethal concentrations. Garlic extracts antagonized the activity of QS receptors LuxR, AhyR and TraR, but were toxic at higher concentrations. P-coumaric acid fully inhibited QS responses of 5999, NTL4 and P. chlororaphis, with no influence on cell viability. Conclusions: The quorum sensing inhibition activity of garlic was extended to novel receptors, and p-coumaric acid was found to possess previously undescribed QS antagonist properties. Significance and Impact of the Study: The results suggest that p-coumaric acid might act as QS inhibitor. Further studies are required to understand its role in the regulation of QS and investigate structurally related compounds.

引用

页码：551 / 555

页数：5

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