Anti-Adipogenic Activity of the Naturally Occurring Phenanthroindolizidine Alkaloid Antofine via Direct Suppression of PPARγ Expression

被引：25

作者：

Jang, Eun Jung ^{[1
,2
]}

Kim, Hyo Kyeong ^{[1
,2
]}

Jeong, Hana ^{[1
,2
]}

Lee, Ye Seul ^{[1
,2
]}

Jeong, Mi Gyeong ^{[1
,2
]}

Bae, Su Jung ^{[1
,2
]}

Kim, Sanghee ^{[3
]}

Lee, Sang Kook ^{[3
]}

Hwang, Eun Sook ^{[1
,2
]}

机构：

[1] Ewha Womans Univ, Coll Pharm, Seoul 120750, South Korea

[2] Ewha Womans Univ, Grad Sch Pharmaceut Sci, Global Res Program Top5, Seoul 120750, South Korea

[3] Seoul Natl Univ, Coll Pharm, Seoul, South Korea

来源：

CHEMISTRY & BIODIVERSITY | 2014年 / 11卷 / 06期

基金：

新加坡国家研究基金会;

关键词：

RISK-FACTOR; ADIPOCYTE DIFFERENTIATION; ADIPOSE-TISSUE; CYNANCHUM-PANICULATUM; CANCER; OBESITY; CELLS; INHIBITION; ANALOGS; TAZ;

D O I：

10.1002/cbdv.201300365

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Antofine (ANTF) is a phenanthroindolizidine alkaloid isolated from the root of Cynanchum paniculatum Kitagawa (Asclepiadaceae), which is used as an herbal remedy for pain and inflammation. ANTF also possesses antiviral and antitumorigenic activities. In this study, we investigated the role of ANTF in adipogenesis. Chronic ABTF administration suppressed adipocyte differentiation and marker expression in a dose-dependent manner. Furthermore, acute administration of ANTF at early stages of differentiation process inhibited lipid droplet formation and adipogenic gene expression. ANTF Treatment decreased expression of PPAR gamma protein, a master transcription factor in the regulation of adipocyte differentiation, leading to a suppression of aP2 promoter activity. These results suggest that ANTF exerts potent anti-adipogenic effects via direct suppression of PPAR gamma protein expression, with consequent downregulation of adipogenic gene expression.

引用

页码：962 / 969

页数：8

共 26 条

[1]

TAZ is required for the osteogenic and anti-adipogenic activities of kaempferol [J].