A Human Proteome Detection and Quantitation Project

被引：150

作者：

Anderson, N. Leigh ^{[1
]}

Anderson, Norman G. ^{[1
]}

Pearson, Terry W. ^{[2
]}

Borchers, Christoph H. ^{[3
]}

Paulovich, Amanda G. ^{[4
]}

Patterson, Scott D. ^{[5
]}

Gillette, Michael ^{[6
,7
]}

Aebersold, Ruedi ^{[8
]}

Carr, Steven A. ^{[6
,7
]}

机构：

[1] Plasma Proteome Inst, Washington, DC 20009 USA

[2] Univ Victoria, Dept Biochem & Microbiol, Victoria, BC V8W 3P6, Canada

[3] Univ Victoria, Genome BC Prote Ctr, Victoria, BC V8Z 7X8, Canada

[4] Fred Hutchinson Canc Res Ctr, Seattle, WA 98109 USA

[5] Amgen Inc, Newbury Pk, CA 91320 USA

[6] MIT, Broad Inst, Cambridge, MA 02142 USA

[7] Harvard Univ, Cambridge, MA 02142 USA

[8] Inst Mol Syst Biol, CH-8093 Zurich, Switzerland

来源：

MOLECULAR & CELLULAR PROTEOMICS | 2009年 / 8卷 / 05期

基金：

美国国家卫生研究院;

关键词：

MASS-SPECTROMETRY; ISOTOPE-DILUTION; PLASMA-PROTEINS; IDENTIFICATION; QUANTIFICATION; ASSAYS;

D O I：

10.1074/mcp.R800015-MCP200

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The lack of sensitive, specific, multiplexable assays for most human proteins is the major technical barrier impeding development of candidate biomarkers into clinically useful tests. Recent progress in mass spectrometry-based assays for proteotypic peptides, particularly those with specific affinity peptide enrichment, offers a systematic and economical path to comprehensive quantitative coverage of the human proteome. A complete suite of assays, e. g. two peptides from the protein product of each of the similar to 20,500 human genes (here termed the human Proteome Detection and Quantitation project), would enable rapid and systematic verification of candidate biomarkers and lay a quantitative foundation for subsequent efforts to define the larger universe of splice variants, post-translational modifications, protein-protein interactions, and tissue localization. Molecular & Cellular Proteomics 8: 883-886, 2009.

引用

页码：883 / 886

页数：4

共 26 条

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