Identification of the beet cyst nematode Heterodera schachtii by PCR

被引：70

作者：

Amiri, S

Subbotin, SA

Moens, M

机构：

[1] State Univ Ghent, Lab Agrozool, B-9000 Ghent, Belgium

[2] Ecole Natl Agr, Dept Phytopathol, Meknes, Morocco

[3] Russian Acad Sci, Inst Parasitol, Moscow 117071, Russia

[4] Agr Res Ctr, Dept Crop Protect, B-9820 Merelbeke, Belgium

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 2002年 / 108卷 / 06期

关键词：

diagnostics; Heterodera betae; H; medicaginis; trifolii; RFLP; species-specific primer;

D O I：

10.1023/A:1019974101225

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

PCR-RFLPs of ITS-rDNA and PCR with species-specific primers were developed for identification of cysts and juveniles of the beet cyst nematode Heterodera schachtii. Restrictions of PCR product by MvaI or ScrFI distinguish H. schachtii, H. betae, H. trifolii and H. medicaginis. RFLP profiles with eight restriction enzymes for these four nematode species are presented. Based on Internal Transcribed Spacer sequences of populations from several Schachtii group species, a specific primer for H. schachtii was designed, permitting amplification of the target sequence from juveniles and cysts of the beet cyst nematode. A duplex PCR protocol tested with a wide range of nematode samples is described.

引用

页码：497 / 506

页数：10

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