Post-translational modifications on proteins: Facile and efficient procedure for the identification of O-glycosylation sites by MALDI-LIFT-TOF/TOF mass spectrometry

被引：31

作者：

Kurogochi, M ^{[1
]}

Matsushita, T ^{[1
]}

Nishimura, SI ^{[1
]}

机构：

[1] Hokkaido Univ, Grad Sch Sci, Div Biol Sci, Frontier Res Ctr Post Gen Sci & Technol, Sapporo, Hokkaido 0010021, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 31期

关键词：

glycosylation; high-throughput screening; mass spectrometry; protein modifications; proteomics;

D O I：

10.1002/anie.200460020

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have LIFT-TOF! Transglycosylation of glycopeptides by β-galactosidase in the presence of lactose combined with tandem mass spectrometry using a 2,5-dihydroxybenzoic acid matrix enables the O-glycosylation sites to be identified (see scheme). The highly sensitive and ideal fragmentation patterns generated from glycopeptide precursor ion peaks can be readily used for precise and reliable structure analyses.

引用

页码：4071 / 4075

页数：5

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