pH-sensitive cyanine dyes for biological applications

被引：71

作者：

Cooper, ME ^{[1
]}

Gregory, S ^{[1
]}

Adie, E ^{[1
]}

Kalinka, S ^{[1
]}

机构：

[1] Amersham Biosci UK Ltd, Bioassays, Cardiff CF14 7YT, S Glam, Wales

来源：

JOURNAL OF FLUORESCENCE | 2002年 / 12卷 / 3-4期

关键词：

fluorescent; cyanine; pH sensitive; confocal microscopy; IN Cell Analyzer; CypHer (TM) 5;

D O I：

10.1023/A:1021366010681

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of functionalized, water-soluble, pH-sensitive pentamethine cyanine (Cy(TM)5) dyes has been designed and synthesized. These probes are fluorescent in acidic media but are non-fluorescent in an alkaline environment. Subtle changes to the structure of these probes can lead to pronounced changes in the pKa of these probes. These probes have been utilized in a cellular environment to detect localized changes in PH using the IN Cell Analyzer, a confocal imager formatted for imaging of cell-based assays.

引用

页码：425 / 429

页数：5

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