Cytoplasmic lipid droplets and mitochondrial distribution in equine oocytes: Implications on oocyte maturation, fertilization and developmental competence after ICSI

被引：71

作者：

Ambruosi, B. ^{[1
]}

Lacalandra, G. M. ^{[1
]}

Lorga, A. I. ^{[1
]}

De Santis, T. ^{[1
]}

Mugnier, S. ^{[2
]}

Matarrese, R. ^{[1
]}

Goudet, G. ^{[2
]}

Dell'Aquila, M. E. ^{[1
]}

机构：

[1] Univ Bari, Dept Anim Prod, I-70010 Bari, Italy

[2] Univ Tours, CNRS, INRA, Physiol Reprod & Comportements UMR85, F-37380 Nouzilly, France

来源：

THERIOGENOLOGY | 2009年 / 71卷 / 07期

关键词：

Equine; Oocyte; Cytoplasmic lipid droplets; Mitochondrial distribution; Intracytoplasmic sperm injection (ICSI); INTRACYTOPLASMIC SPERM INJECTION; INITIAL CHROMATIN CONFIGURATION; EARLY EMBRYONIC-DEVELOPMENT; IN-VITRO FERTILIZATION; HORSE OOCYTES; MEIOTIC COMPETENCE; CUMULUS MORPHOLOGY; ADENOSINE-TRIPHOSPHATE; BOVINE OOCYTES; PIG OOCYTES;

D O I：

10.1016/j.theriogenology.2008.12.002

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Lipid droplets (LDs) and mitochondria in the ooplasm are essential for energy production required for maturation, fertilization and embryo development. This Study investigates the correlations between cytoplasmic LDs polar aggregation and: (1) nuclear maturation (Experiment 1): (2) mitochondrial (nit) distribution pattern and localization (Experiment 2): (3) fertilization and embryonic development after intracytoplasmic sperm injection (ICSI; Experiment 3) in equine oocytes recovered from slaughtered mares and matured in vitro. Morphologically normal oocytes were selected after culture and categorized as having polar (P) aggregation or uniform (U) distribution of LDs. In Experiment 1. the maturation rate was significantly higher in P compared with U oocytes (69%, 40/58 vs. 32%, 13/41: P < 0.001). In Experiment 2. it was observed that P and U oocytes showed heterogeneous mt distribution at comparable rates (68%, 25/37 vs. 50%, 2/4 for P and U respectively; NS). Moreover, only in 8/25 (32%) of P oocytes. LDs overlapped with nit aggregates in the area containing meiotic spindle. In Experiment 3. normal fertilization (51%, 19/37 vs. 60%. 6/10. for P and U) and cleavage rates (83%, 20/24 vs. 67%, 4/6, for P and U) did not differ between groups, also in oocytes with LDs located nearby the polar body. Overall, P aggregation of LDs was related to cumulus of expansion at collection. In conclusion, in equine matured oocytes. P aggregation of LDs is related with cumulus expansion and nuclear maturation. However, it is not related with heterogeneous mt distribution and cannot be considered a predictive indicator for normal fertilization and embryo

引用

页码：1093 / 1104

页数：12

共 56 条

[1]

Accumulation of cytoplasmic lipid droplets in bovine embryos and cryotolerance of embryos developed in different culture systems using serum-free or serum-containing media [J].