DNA affinity capture and protein profiling by SELDI-TOF mass spectrometry: effect of DNA methylation

被引：20

作者：

Bane, TK

LeBlanc, JF

Lee, TD

Riggs, AD

机构：

[1] City Hope Natl Med Ctr, Beckman Res Inst, Div Biol, Duarte, CA 91010 USA

[2] City Hope Natl Med Ctr, Beckman Res Inst, Div Immunol, Duarte, CA 91010 USA

[3] Ciphergen Biosyst, Fremont, CA 94555 USA

来源：

NUCLEIC ACIDS RESEARCH | 2002年 / 30卷 / 14期

关键词：

D O I：

10.1093/nar/gnf068

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here that surface enhanced laser desorption/ionization-time of flight (SELDI-TOF) mass spectrometry, as performed on a Ciphergen Biosystems ProteinChip System, can be used in conjunction with DNA affinity capture (DACA) to study specific DNA-protein binding. Using DNA molecules bound to a surface, sequence-specific Interactions can be detected as demonstrated by a mutation affecting the binding profile for TBP, a transcription factor. Also, a comparison between methylated and unmethylated promoter-containing DNA fragments shows numerous binding profile differences over a mass range extending to >60 kDa. The binding of several proteins Is Inhibited by methylation of the DNA.

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页数：6

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