Effect of pretreatment chemicals on xylose fermentation by Pichia stipitis

被引:40
作者
Agbogbo, Frank K. [1 ]
Wenger, Kevin S. [1 ]
机构
[1] Novozymes N Amer Inc, Franklinton, NC 27525 USA
关键词
ammonium sulphate; calcium sulphate; ethanol; Pichia stipitis; sodium sulphate; xylose;
D O I
10.1007/s10529-006-9192-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Pretreatment of biomass with dilute H2SO4 results in residual acid which is neutralized with alkalis such as Ca(OH)(2), NaOH and NH4OH. The salt produced after neutralization has an effect on the fermentation of Pichia stipitis. Synthetic media of xylose (60 g total sugar/l) was fermented to ethanol in the presence and absence of the salts using P. stipitis CBS 6054. CaSO4 enhanced growth and xylitol production, but produced the lowest ethanol concentration and yield after 140 h. Na2SO4 inhibited xylitol production, slightly enhanced growth towards the end of fermentation but had no significant effect on xylose consumption and ethanol concentration. (NH4)(2)SO4 inhibited growth, had no effect on xylitol production, and enhanced xylose consumption and ethanol production.
引用
收藏
页码:2065 / 2069
页数:5
相关论文
共 16 条
[1]   Ammonium hydroxide detoxification of spruce acid hydrolysates [J].
Alriksson, B ;
Horváth, IS ;
Sjöde, A ;
Nilvebrant, NO ;
Jönsson, LJ .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2005, 121 :911-922
[2]  
Blomberg A, 2000, FEMS MICROBIOL LETT, V182, P1
[3]   Effects of lignocellulose degradation products on ethanol fermentations of glucose and xylose by Saccharomyces cerevisiae, Zymomonas mobilis, Pichia stipitis, and Candida shehatae [J].
Delgenes, JP ;
Moletta, R ;
Navarro, JM .
ENZYME AND MICROBIAL TECHNOLOGY, 1996, 19 (03) :220-225
[4]   XYLOSE FERMENTATION BY YEASTS .3. COMPARISON OF PACHYSOLEN-TANNOPHILUS AND PICHIA-STIPITIS [J].
DELLWEG, H ;
RIZZI, M ;
METHNER, H ;
DEBUS, D .
BIOTECHNOLOGY LETTERS, 1984, 6 (06) :395-400
[5]   THE FERMENTATION OF HEXOSE AND PENTOSE SUGARS BY CANDIDA-SHEHATAE AND PICHIA-STIPITIS [J].
DUPREEZ, JC ;
BOSCH, M ;
PRIOR, BA .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1986, 23 (3-4) :228-233
[6]   Comparison of different pretreatments in ethanol fermentation using corn cob hemicellulosic hydrolysate with Pichia stipitis and Candida shehatae [J].
Eken-Saraçoglu, N ;
Arslan, Y .
BIOTECHNOLOGY LETTERS, 2000, 22 (10) :855-858
[7]   Relative fermentability of lignocellulosic dilute-acid prehydrolysates -: Application of a Pichia stipitis-based toxicity assay [J].
Fenske, JJ ;
Hashimoto, A ;
Penner, MH .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 1998, 73 (2-3) :145-157
[8]   ANTAGONISM BETWEEN GROWTH AND FLOCCULATION IN PICHIA-STIPITIS NRRL Y-7124 - INFLUENCE OF CA+2 AND MG+2 IONS [J].
GUEBEL, DV ;
NUDEL, CB .
BIOTECHNOLOGY LETTERS, 1994, 16 (02) :143-148
[9]   INFLUENCE OF THE NITROGEN-SOURCE ON GROWTH AND ETHANOL-PRODUCTION BY PICHIA-STIPITIS NRRL Y-7124 [J].
GUEBEL, DV ;
CORDENONS, A ;
CASCONE, O ;
GIULIETTI, AM ;
NUDEL, C .
BIOTECHNOLOGY LETTERS, 1992, 14 (12) :1193-1198
[10]   Ethanol production from wheat straw hemicellulose hydrolysate by Pichia stipitis [J].
Nigam, JN .
JOURNAL OF BIOTECHNOLOGY, 2001, 87 (01) :17-27