Molecular analysis of Clostridium difficile isolates recovered from horses with diarrhea

被引：41

作者：

Arroyo, Luis G. ^{[1
]}

Staempfli, Henry ^{[1
]}

Weese, J. Scott ^{[1
]}

机构：

[1] Univ Guelph, Dept Clin Studies, Ontario Vet Coll, Guelph, ON N1G 2W1, Canada

来源：

VETERINARY MICROBIOLOGY | 2007年 / 120卷 / 1-2期

关键词：

Clostridium difficile; horse; colitis; typing; toxins; genes;

D O I：

10.1016/j.vetmic.2006.10.013

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Clostridium difficile is an important cause of diarrhea in horses, causing sporadic and epidemic disease of varying severity. This study evaluated the molecular characteristics of 48 C. difficile isolates recovered from diarrheic horses admitted to a veterinary hospital by using PCR-ribotyping and toxin gene profile. Additionally, feces were tested for the presence of C. difficile toxin A/B via enzyme immunosorbant assay (EIA) in 38 horses. The toxin genes tcdA, tcdB and cdtB were present in 27 (56.25%), 35 (72.91%) and 2 (4.1%) strains, respectively. Eight isolates (16.6%) were A(-)B(+) variants. Thirteen of forty-eight isolates (27.0%) did not posses any toxin genes (A(-)B(-)CDT(-)). A positive EIA result was reported in 17 (44%) of the cases. There was no association between the presence of different ribotypes or strains and toxin gene(s) profiles and the clinical outcome. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：179 / 183

页数：5

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