The relevance of the phosphatidylinositolphosphat-binding motif FRRGT of Atg18 and Atg21 for the Cvt pathway and autophagy

被引：73

作者：

Krick, Roswitha ^{[1
]}

Tolstrup, Joern ^{[1
]}

Appelles, Anika ^{[1
]}

Henke, Sandra ^{[1
]}

Thumm, Michael ^{[1
]}

机构：

[1] Univ Gottingen, Ctr Biochem & Mol Cell Biol, D-37073 Gottingen, Germany

来源：

FEBS LETTERS | 2006年 / 580卷 / 19期

关键词：

Cvt pathway; proaminopeptidase I; autophagy; phosphatidylinositolphosphate; lipid binding; FRRGT motif;

D O I：

10.1016/j.febslet.2006.07.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Atg18 and Atg21 are homologous S. cerevisiae autophagy proteins. Atg18 is essential for biogenesis of Cvt vesicles and autophagosomes, while Atg21 is only essential for Cvt vesicle formation. We found that mutated Atg18-(FTTGT), which lost almost completely its binding to PtdIns3P and PtdIns(3,5)P-2, is non-functional during the Cvt pathway but active during autophagy and pexophagy. Since the Cvt pathway does not depend on PtdIns(3,5)P-2, we conclude that the Cvt pathway requires binding of Atg18 to PtdIns3P. Mutated Atg21-(FTTGT) is inactive during the Cvt pathway but showed only partly reduced binding to PtdIns-phosphates, suggesting further lipid binding domains in Atg21. GFP-Atg18-(FTTGT) and Atg21-(FTTGT)-GFP are released from vacuolar punctae to the cytosol. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4632 / 4638

页数：7

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