Cell death in Leishmania induced by stress and differentiation:: programmed cell death or necrosis?

被引:132
作者
Zangger, H
Mottram, JC
Fasel, N
机构
[1] Univ Lausanne, Inst Biochem, CH-1066 Epalinges, Switzerland
[2] Univ Glasgow, Wellcome Ctr Mol Parasitol, Glasgow G11 6NU, Lanark, Scotland
关键词
Leishmania; programmed cell death; necrosis; DNA; fragmentation; cysteine proteases; host-parasite interaction;
D O I
10.1038/sj.cdd.4401071
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Unicellular organisms, such as the protozoan parasite Leishmania, can be stimulated to show some morphological and biochemical features characteristic of mammalian apoptosis. This study demonstrates that under a variety of stress conditions such as serum deprivation, heat shock and nitric oxide, cell death can be induced leading to genomic DNA fragmentation into oligonucleosomes. DNA fragmentation was observed, without induction, in the infectious stages of the parasite, and correlated with the presence of internucleosomal nuclease activity, visualisation of 45 to 59 kDa nucleases and detection of TUNEL-positive nuclei. DNA fragmentation was not dependent on active effector downstream caspases nor on the lysosomal cathepsin L-like enyzmes CPA and CPB. These data are consistent with the presence of a caspase-independent cell death mechanism in Leishmania, induced by stress and differentiation that differs significantly from metazoa.
引用
收藏
页码:1126 / 1139
页数:14
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