Rapid identification and differentiation of clinical isolates of enteropathogenic Escherichia coli (EPEC), atypical EPEC, and Shiga toxin-producing Escherichia coli by a one-step multiplex PCR method

被引：28

作者：

Mueller, Daniel

Hagedorn, Peter

Brast, Sabine

Heusipp, Gerhard

Bielaszewska, Martina

Friedrich, Alexander W.

Karch, Helge

Schmidt, M. Alexander

机构：

[1] Univ Klinikum Munster, Zentrum Mol Biol Entzundung, Inst Infektiol, Munster, Germany

[2] Univ Klinikum Munster, Inst Hyg, D-48149 Munster, Germany

[3] Univ Klinikum Munster, Natl Konsiliarlab Hamolyt Uram Syndrom, D-48149 Munster, Germany

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2006年 / 44卷 / 07期

关键词：

D O I：

10.1128/JCM.00895-06

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx(1), and stx(2).

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页码：2626 / 2629

页数：4

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