Analysis of biogenic amines by solid-phase microextraction and high-performance liquid chromatography with electrochemical detection

被引：24

作者：

Auger, J ^{[1
]}

Boulay, R

Jaillais, B

Delion-Vancassel, S

机构：

[1] Univ Tours, Inst Rech Biol Insecte, F-37200 Tours, France

[2] Univ Tours, Lab Ethol & Pharmacol Comportement, F-37200 Tours, France

来源：

JOURNAL OF CHROMATOGRAPHY A | 2000年 / 870卷 / 1-2期

关键词：

extraction methods; solid-phase microextraction; biogenic amines; dopamine; serotonin;

D O I：

10.1016/S0021-9673(99)01077-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the new solid-phase microextraction method by high-performance liquid chromatography with electrochemical detection for the analysis of biogenic amines. The Carbowax-Templated Resin 50 mu m (purple) fibre coating offers good performances for dopamine and serotonin separation, i.e., good selectivity and high sensibility (0.1 mu g l(-1)). We also tested this fibre for biogenic amines quantification of rat striatum, The coating seems to be selective towards the amines and has low affinity for the metabolites, allowing a good separation and preventing drawbacks from the biological matrix. These first results obtained using this original separation method offer large perspectives of application to many biological samples. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：395 / 403

页数：9

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