PLD2 is enriched on exosomes and its activity is correlated to the release of exosomes

被引：183

作者：

Laulagnier, K

Grand, D

Dujardin, A

Hamdi, S

Vincent-Schneider, H

Lankar, D

Salles, JP

Bonnerot, C

Perret, B

Record, M

机构：

[1] CHU Purpan, INSERM, U563, Dept Lipoprot & Mediateurs Lipidiques,CPTP, F-31024 Toulouse 3, France

[2] Inst Curie, INSERM U520, F-75248 Paris 05, France

来源：

FEBS LETTERS | 2004年 / 572卷 / 1-3期

关键词：

D O I：

10.1016/j.febslet.2004.06.082

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Exosomes are small vesicles secreted by different immune cells and which display anti-tumoral properties. Stimulation of RBL-2H3 cells with ionomycin triggered phospholipase D2 (PLD2) translocation from plasma membrane to intracellular compartments and the release of exosomes. Although exosomes; carry the two isoforms of PLD, PLD2 was enriched and specifically sorted on exosomes when overexpressed in cells. PLD activity present on exosomes was clearly increased following PLD2 overexpression. PLD2 activity in cells was correlated to the amount of exosome released, as measured by FACS. Therefore, the present work indicates that exosomes can vehicle signaling enzymes. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：11 / 14

页数：4

共 23 条

[1] Phospholipase D activity facilitates Ca2+-induced aggregation and fusion of complex liposomes [J].