Optimization of nutrients for gellan gum production by Sphingomonas paucimobilis ATCC-31461 in molasses based medium using response surface methodology

被引：113

作者：

Banik, R. M. ^{[1
]}

Santhiagu, A.

Upadhyay, S. N.

机构：

[1] Banaras Hindu Univ, Inst Technol, Sch Biochem Engn, Varanasi 221005, Uttar Pradesh, India

[2] Banaras Hindu Univ, Inst Technol, Dept Chem Engn, Varanasi 221005, Uttar Pradesh, India

来源：

BIORESOURCE TECHNOLOGY | 2007年 / 98卷 / 04期

关键词：

gellan; exopolysaccharide; molasses; Sphingomonas paucimobilis; central composite design; response surface methodology;

D O I：

10.1016/j.biortech.2006.03.012

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

A molasses based medium for the production of gellan by Sphingomonas paucimobilis ATCC-31461 was developed. Placket-Burman design criterion was applied to study the effect of various nutrient supplements on gellan production using molasses. Among the 20 variables tested, molasses, tryptone, casaminoacid, disodium hydrogen orthophosphate and manganese chloride showed significant effect on gellan production. A central composite design was applied to determine the optimum concentrations of the significant variables obtained from Placket-Burman design. Most suitable medium composition for production of gellan was (g/l): molasses-112.5; tryptone-1; casaminoacid-1; disodium. hydrogen orthophosphate-1; manganese chloride-0.947 and the optimum gellan production was 13.814 g/l. (c) 2006 Elsevier Ltd. All rights reserved.

引用

页码：792 / 797

页数：6

共 19 条

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