Immobilization of Flavonol 3-O-β-Heterodisaccharidase on Porous Glass and Production of Rutinose from Rutin

被引：2

作者：

Coniglio, Marcela ^{[1
]}

Kreis, Wolfgang ^{[1
]}

机构：

[1] Univ Erlangen Nurnberg, Lehrstuhl Pharmazeut Biol, Dept Biol, D-91058 Erlangen, Germany

来源：

PLANTA MEDICA | 2009年 / 75卷 / 13期

关键词：

Fagopyrum esculentum; Polygonaceae; enzyme immobilization; disaccharidase; rutin; rutinose; HIGH-FAT DIET; FAGOPYRUM-ESCULENTUM; RABBITS; HERB;

D O I：

10.1055/s-0029-1185703

中图分类号：

Q94 [植物学];

学科分类号：

071001 [植物学];

摘要：

Flavonol 3-O-beta-heterodisaccharidase (FHG 1) was isolated from the dried herb of Fagopyrum esculentum, immobilized on Porous glass, and used for the release of rutinose from rutin. The stability of the enzyme in its free or immobilized form was observed continuously at two different temperatures (4 degrees C and 25 degrees C). T-1/2 values were determined to be about 48 h for the free enzyme and about 300 h for the immobilized enzyme. The rutinose released was isolated by fractionated ethanol precipitation.

引用

页码：1459 / 1461

页数：3

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