Single-Molecule Assay of Biological Reaction in Femtoliter Chamber Array

被引：9

作者：

Iino, Ryota ^{[1
]}

Lam, Liza ^{[1
]}

Tabata, Kazuhito V. ^{[1
]}

Rondelez, Yannick ^{[2
]}

Noji, Hiroyuki ^{[1
]}

机构：

[1] Osaka Univ, Inst Sci & Ind Res, Osaka 5670047, Japan

[2] Univ Tokyo, Inst Ind Sci, Meguro Ku, Tokyo 1538505, Japan

来源：

JAPANESE JOURNAL OF APPLIED PHYSICS | 2009年 / 48卷 / 08期

关键词：

ATP SYNTHASE; CATALYTIC SUBUNITS; F-1-ATPASE; ROTATION; DNA; MANIPULATION; RESOLUTION; CLEAVAGE; MACHINE; STEPS;

D O I：

10.1143/JJAP.48.08JA04

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

We previously developed micron-sized, femtoliter (fL) reaction chamber array made of polydimethylsiloxane and polyacrylamide. Downsizing of the reaction volume to fL level made it possible to carry out single-molecule assay (SMA) of biological reactions in a straightforward manner. In this review, preparation of the fL chamber array and its application to SMA of enzyme, DNA, and rotary motor protein under an optical microscope are described. (C) 2009 The Japan Society of Applied Physics

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页数：5

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